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dc.contributor.author
Betts, Alex
dc.contributor.author
Vasse, Marie
dc.contributor.author
Kaltz, Oliver
dc.contributor.author
Hochberg, Michael E.
dc.date.accessioned
2018-10-22T12:27:51Z
dc.date.available
2018-10-19T14:29:39Z
dc.date.available
2018-10-22T12:27:51Z
dc.date.issued
2013-11
dc.identifier.issn
1752-4571
dc.identifier.issn
1752-4563
dc.identifier.other
10.1111/eva.12085
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/297624
dc.identifier.doi
10.3929/ethz-b-000297624
dc.description.abstract
Antibiotic resistance is becoming increasingly problematic for the treatment of infectious disease in both humans and livestock. The bacterium Pseudomonas aeruginosa is often found to be resistant to multiple antibiotics and causes high patient mortality in hospitals. Bacteriophages represent a potential option to combat pathogenic bacteria through their application in phage therapy. Here, we capitalize on previous studies showing how evolution may increase phage infection capacity relative to ancestral genotypes. We passaged four different phage isolates (podoviridae, myoviridae) through six serial transfers on the ancestral strain of Pseudomonas aeruginosa PAO1. We first demonstrate that repeated serial passage on ancestral bacteria increases infection capacity of bacteriophage on ancestral hosts and on those evolved for one transfer. This result is confirmed when examining the ability of evolved phage to reduce ancestral host population sizes. Second, through interaction with a single bacteriophage for 24 h, P. aeruginosa can evolve resistance to the ancestor of that bacteriophage; this also provides these evolved bacteria with cross‐resistance to the other three bacteriophages. We discuss how the evolutionary training of phages could be employed as effective means of combatting bacterial infections or disinfecting surfaces in hospital settings, with reduced risk of bacterial resistance compared with conventional methods.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
coevolution
en_US
dc.subject
cross-infection
en_US
dc.subject
cystic fibrosis
en_US
dc.subject
disinfection
en_US
dc.subject
evolutionary engineering
en_US
dc.subject
experimental evolution
en_US
dc.subject
nosocomial
en_US
dc.subject
phage therapy
en_US
dc.subject
phage training
en_US
dc.subject
resistance
en_US
dc.title
Back to the future: evolving bacteriophages to increase their effectiveness against the pathogenPseudomonas aeruginosaPAO1
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
dc.date.published
2013-07-15
ethz.journal.title
Evolutionary Applications
ethz.journal.volume
6
en_US
ethz.journal.issue
7
en_US
ethz.journal.abbreviated
Evol. appl.
ethz.pages.start
1054
en_US
ethz.pages.end
1063
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
Hoboken, NJ
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
::ETH Zürich::00002 - ETH Zürich
en_US
ethz.date.deposited
2018-10-19T14:29:40Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-10-22T12:27:55Z
ethz.rosetta.lastUpdated
2018-12-03T01:33:20Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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