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dc.contributor.author
Scherrer, Tanja
dc.contributor.author
Femmer, Christian
dc.contributor.author
Schiess, Ralph
dc.contributor.author
Aebersold, Ruedi
dc.contributor.author
Gerber, André P.
dc.date.accessioned
2019-04-11T13:44:51Z
dc.date.available
2017-06-09T09:18:07Z
dc.date.available
2019-04-11T13:13:51Z
dc.date.available
2019-04-11T13:44:51Z
dc.date.issued
2011
dc.identifier.issn
1474-7596
dc.identifier.issn
1465-6906
dc.identifier.issn
1474-760X
dc.identifier.issn
1465-6914
dc.identifier.other
10.1186/gb-2011-12-1-r3
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/29988
dc.identifier.doi
10.3929/ethz-b-000029988
dc.description.abstract
Background Glucose inhibition of gluconeogenic growth suppressor 2 protein (Gis2p) and zinc-finger protein 9 (ZNF9) are conserved yeast and human zinc-finger proteins. The function of yeast Gis2p is unknown, but human ZNF9 has been reported to bind nucleic acids, and mutations in the ZNF9 gene cause the neuromuscular disease myotonic dystrophy type 2. To explore the impact of these proteins on RNA regulation, we undertook a systematic analysis of the RNA targets and of the global implications for gene expression. Results Hundreds of mRNAs were associated with Gis2p, mainly coding for RNA processing factors, chromatin modifiers and GTPases. Target mRNAs contained stretches of G(A/U)(A/U) trinucleotide repeats located in coding sequences, which are sufficient for binding to both Gis2p and ZNF9, thus implying strong structural conservation. Predicted ZNF9 targets belong to the same functional categories as seen in yeast, indicating functional conservation, which is further supported by complementation of the large cell-size phenotype of gis2 mutants with ZNF9. We further applied a matched-sample proteome-transcriptome analysis suggesting that Gis2p differentially coordinates expression of RNA regulons, primarily by reducing mRNA and protein levels of genes required for ribosome assembly and by selectively up-regulating protein levels of myosins. Conclusions This integrated systematic exploration of RNA targets for homologous RNA-binding proteins indicates an unexpectedly high conservation of the RNA-binding properties and of potential targets, thus predicting conserved RNA regulons. We also predict regulation of muscle-specific genes by ZNF9, adding a potential link to the myotonic dystrophy related phenotypes seen in ZNF9 mouse models.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
Gene Ontology
en_US
dc.subject
Synthetic Complete
en_US
dc.subject
Saccharomyces Genome Database
en_US
dc.subject
Stanford Microarray Database
en_US
dc.subject
mRNA Fate
en_US
dc.title
Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
dc.date.published
2011-01-13
ethz.journal.title
Genome Biology
ethz.journal.volume
12
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Genome biol.
ethz.pages.start
R3
en_US
ethz.size
19 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004405180
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
ethz.date.deposited
2017-06-09T09:18:14Z
ethz.source
ECIT
ethz.identifier.importid
imp59364da206a6b97593
ethz.ecitpid
pub:49524
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T15:00:28Z
ethz.rosetta.lastUpdated
2019-04-11T13:45:20Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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