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dc.contributor.author
Zhang, Yifan
dc.contributor.author
Moeller, Ralf
dc.contributor.author
Meneses, Nicolás
dc.contributor.author
Tran, Sophia
dc.contributor.author
Dubovcova, Barbora
dc.contributor.author
Drissner, David
dc.contributor.author
Mathys, Alexander
dc.date.accessioned
2019-02-14T16:30:41Z
dc.date.available
2018-11-01T15:45:49Z
dc.date.available
2018-11-14T11:09:22Z
dc.date.available
2019-02-14T16:30:41Z
dc.date.issued
2018-11
dc.identifier.issn
1664-302X
dc.identifier.other
10.3389/fmicb.2018.02720
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/300651
dc.identifier.doi
10.3929/ethz-b-000300651
dc.description.abstract
Low energy electron beam (LEEB) treatment is an emerging non-thermal technology that performs surface decontamination with a minimal influence on food quality. Bacterial spore resistance toward LEEB treatment and its influencing factors were investigated in this study. Spores from Geobacillus and Bacillus species were treated with a lab-scale LEEB at energy levels of 80 and 200 keV. The spore resistances were expressed as D-values (the radiation dose required for one log10 reduction at a given energy level) calculated from the linear regression of log10 reduction against absorbed dose of the sample. The results revealed that the spore inactivation efficiency by LEEB is comparable to that of other ionizing radiations and that the inactivation curves are mostly log10-linear at the investigated dose range (3.8 – 8.2 kGy at 80 keV; 6.0 – 9.8 kGy at 200 keV). The D-values obtained from the wildtype strains varied from 2.2 – 3.0 kGy at 80 keV, and from 2.2 – 3.1 kGy at 200 keV. Bacillus subtilis mutant spores lacking α/β-type small, acid-soluble spore proteins showed decreased D-values (1.3 kGy at 80 and 200 keV), indicating that spore DNA is one of the targets for LEEB spore inactivation. The results revealed that bacterial species, sporulation conditions and the treatment dose influence the spore LEEB inactivation. This finding indicates that for the application of this emerging technology, special attention should be paid to the choice of biological indicator, physiological state of the indicator and the processing settings. High spore inactivation efficiency supports the application of LEEB for the purpose of food surface decontamination. With its environmental, logistical, and economic advantages, LEEB can be a relevant technology for surface decontamination to deliver safe, minimally processed and additive-free food products.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Bacterial spore
en_US
dc.subject
Low energy electron beam
en_US
dc.subject
Inactivation
en_US
dc.subject
influencing factors
en_US
dc.subject
Surface decontamination
en_US
dc.subject
Ionizing radiation
en_US
dc.subject
DNA Damage
en_US
dc.title
Geobacillus and Bacillus spore inactivation by low energy electron beam technology
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.title.subtitle
Resistance and influencing factors
en_US
ethz.journal.title
Frontiers in Microbiology
ethz.journal.volume
9
en_US
ethz.journal.abbreviated
Front. microbiol.
ethz.pages.start
2720
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09571 - Mathys, Alexander / Mathys, Alexander
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09571 - Mathys, Alexander / Mathys, Alexander
en_US
ethz.date.deposited
2018-11-01T15:46:02Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-02-14T16:30:58Z
ethz.rosetta.lastUpdated
2019-02-14T16:30:58Z
ethz.rosetta.versionExported
true
ethz.COinS
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