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dc.contributor.author
Mattei, Simone
dc.contributor.author
Tan, Aaron
dc.contributor.author
Glass, Bärbel
dc.contributor.author
Müller, Barbara
dc.contributor.author
Kräusslich, Hans-Georg
dc.contributor.author
Briggs, John A.G.
dc.date.accessioned
2018-11-28T09:48:28Z
dc.date.available
2018-11-09T05:15:37Z
dc.date.available
2018-11-28T09:48:28Z
dc.date.issued
2018-09-14
dc.identifier.issn
0027-8424
dc.identifier.issn
1091-6490
dc.identifier.other
10.1073/pnas.1811237115
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/302323
dc.identifier.doi
10.3929/ethz-b-000302323
dc.description.abstract
HIV-1 maturation occurs via multiple proteolytic cleavages of the Gag polyprotein, causing rearrangement of the virus particle required for infectivity. Cleavage results in beta-hairpin formation at the N terminus of the CA (capsid) protein and loss of a six-helix bundle formed by the C terminus of CA and the neighboring SP1 peptide. How individual cleavages contribute to changes in protein structure and interactions, and how the mature, conical capsid forms, are poorly understood. Here, we employed cryoelectron tomography to determine morphology and high-resolution CA lattice structures for HIV-1 derivatives in which Gag cleavage sites are mutated. These analyses prompt us to revise current models for the crucial maturation switch. Unlike previously proposed, cleavage on either terminus of CA was sufficient, in principle, for lattice maturation, while complete processing was needed for conical capsid formation. We conclude that destabilization of the six-helix bundle, rather than beta-hairpin formation, represents the main determinant of structural maturation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
National Academy of Sciences
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
retrovirus
en_US
dc.subject
capsid
en_US
dc.subject
cryoelectron tomography
en_US
dc.subject
maturation
en_US
dc.subject
subtomogram averaging
en_US
dc.title
High-resolution structures of HIV-1 Gag cleavage mutants determine structural switch for virus maturation
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2018-10-02
ethz.journal.title
Proceedings of the National Academy of Sciences of the United States of America
ethz.journal.volume
115
en_US
ethz.journal.issue
40
en_US
ethz.journal.abbreviated
PNAS
ethz.pages.start
E9401
en_US
ethz.pages.end
E9410
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2018-11-09T05:15:40Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-11-28T09:48:35Z
ethz.rosetta.lastUpdated
2018-11-28T09:48:35Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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