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Leparc, German G.
Pires, Nuno D.
Lidell, Martin E.
Virtanen, Kirsi A.
- Journal Article
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Rights / licenseCreative Commons Attribution 4.0 International
Recruitment and activation of thermogenic adipocytes have received increasing attention as a strategy to improve systemic metabolic control. The analysis of brown and brite adipocytes is complicated by the complexity of adipose tissue biopsies. Here, we provide an in-depth analysis of pure brown, brite, and white adipocyte transcriptomes. By combining mouse and human transcriptome data, we identify a gene signature that can classify brown and white adipocytes in mice and men. Using a machine-learning-based cell deconvolution approach, we develop an algorithm proficient in calculating the brown adipocyte content in complex human and mouse biopsies. Applying this algorithm, we can show in a human weight loss study that brown adipose tissue (BAT) content is associated with energy expenditure and the propensity to lose weight. This online available tool can be used for in-depth characterization of complex adipose tissue samples and may support the development of therapeutic strategies to increase energy expenditure in humans Show more
Journal / seriesCell Reports
Pages / Article No.
Subjectpure adipocyte populations; gene signature; deconvolution; BAT content
Organisational unit03819 - Wolfrum, Christian / Wolfrum, Christian
02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
162887 - Analysis of brite adipocyte formation and function (SNF)
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