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dc.contributor.author
Tayyrov, Annageldi
dc.contributor.author
Schmieder, Stefanie S.
dc.contributor.author
Bleuler-Martinez, Silvia
dc.contributor.author
Plaza, David F.
dc.contributor.author
Künzler, Markus
dc.date.accessioned
2018-12-04T17:31:25Z
dc.date.available
2018-11-30T05:40:28Z
dc.date.available
2018-12-04T17:31:25Z
dc.date.issued
2018-12
dc.identifier.other
10.1128/AEM.02051-18
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/307563
dc.identifier.doi
10.3929/ethz-b-000307563
dc.description.abstract
Resistance of fungi to predation is thought to be mediated by toxic metabolites and proteins. Many of these fungal defense effectors are highly abundant in the fruiting body and not produced in the vegetative mycelium. The defense function of fruiting body-specific proteins, however, including cytoplasmically localized lectins and antinutritional proteins such as biotin-binding proteins, is mainly based on toxicity assays using bacteria as a heterologous expression system, with bacterivorous/omnivorous model organisms as predators. Here, we present an ecologically more relevant experimental setup to assess the toxicity of potential fungal defense proteins towards the fungivorous, stylet-feeding nematodes Aphelenchus avenae and Bursaphelenchus okinawaensis. As a heterologous expression host, we exploited the filamentous fungus Ashbya gossypii. Using this new system, we assessed the toxicity of six previously characterized, cytoplasmically localized, potential defense proteins from fruiting bodies of different fungal phyla against the two fungivorous nematodes. We found that all of the tested proteins were toxic against both nematodes, albeit to various degrees. The toxicity of these proteins against both fungivorous and bacterivorous nematodes suggests that their targets have been conserved between the different feeding groups of nematodes and that bacterivorous nematodes are valid model organisms to assess the nematotoxicity of potential fungal defense proteins.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Microbiology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Mycophagy
en_US
dc.subject
Lectin
en_US
dc.subject
Avidin
en_US
dc.subject
Filamentous fungus
en_US
dc.subject
Ashbya gossypii
en_US
dc.subject
Nematotoxicity
en_US
dc.title
Toxicity of Potential Fungal Defense Proteins towards the Fungivorous Nematodes Aphelenchus avenae and Bursaphelenchus okinawaensis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2018-11-15
ethz.journal.title
Applied and Environmental Microbiology
ethz.journal.volume
84
en_US
ethz.journal.issue
23
en_US
ethz.pages.start
e02051
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Molecular dissection of the chemical defense system of multicellular fungi against predation
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.pubmed
30242007
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03408 - Aebi, Markus / Aebi, Markus
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::08838 - Künzler, Markus (Tit.-Prof.)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03408 - Aebi, Markus / Aebi, Markus
ethz.grant.agreementno
173097
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
ethz.date.deposited
2018-11-30T05:40:31Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-12-04T17:31:59Z
ethz.rosetta.lastUpdated
2019-02-03T12:01:16Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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