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We present an automated platform to perform enzymatic assays in nanoliter droplet arrays with multimodal readout. We analyzed the animal feed additive phytase, an industrially-relevant enzyme, which was expressed and secreted by recombinant yeast cells encapsulated in droplets. The relative enzyme concentration could be determined by time-lapse fluorescence spectroscopy. Moreover, the progress of the multistep phytase-catalyzed reaction of non-fluorogenic substrates was directly monitored by mass spectrometry (MS), without the need of further purification. Show more
Book title22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectDroplet arrays; MALDI-MS; Phytase secretion
Organisational unit03807 - Dittrich, Petra / Dittrich, Petra
NotesExtended abstract. Conference lecture on November 15, 2018.
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