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We present an automated platform to perform enzymatic assays in nanoliter droplet arrays with multimodal readout. We analyzed the animal feed additive phytase, an industrially-relevant enzyme, which was expressed and secreted by recombinant yeast cells encapsulated in droplets. The relative enzyme concentration could be determined by time-lapse fluorescence spectroscopy. Moreover, the progress of the multistep phytase-catalyzed reaction of non-fluorogenic substrates was directly monitored by mass spectrometry (MS), without the need of further purification. Show more
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Book title22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTas 2018)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectDroplet arrays; MALDI-MS; Phytase secretion
Organisational unit03807 - Dittrich, Petra / Dittrich, Petra
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NotesExtended abstract. Conference lecture on November 15, 2018.
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