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We present a microelectrode platform, integrated on a microfluidic chip, to study electrofusion of giant lipid vesicles with smaller vesicles, such as large liposomes made of synthetic lipids and vesicles derived from HEK293T cells. We monitored the fusion through lipid mixing, specifically the transfer of labeled lipids onto the giant vesicle membrane. The method described here represents a key step towards the creation of hybrid vesicles and is ultimately valuable for the bottom-up creation of artificial cells. Show more
Book title22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectArtificial cells; Lipid vesicles; Cell-derived vesicles; Membrane fusion
Organisational unit03807 - Dittrich, Petra / Dittrich, Petra
NotesPoster abstract. Poster presentation on November 12, 2018.
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