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dc.contributor.author
Martini, Francesca
dc.contributor.author
Eckmair, Barbara
dc.contributor.author
Štefanić, Saša
dc.contributor.author
Jin, Chunsheng
dc.contributor.author
Garg, Monika
dc.contributor.author
Yan, Shi
dc.contributor.author
Jiménez-Castells, Carmen
dc.contributor.author
Hykollari, Alba
dc.contributor.author
Neupert, Christine
dc.contributor.author
Venco, Luigi
dc.contributor.author
Silva, Daniel Varón
dc.contributor.author
Wilson, Iain B.H.
dc.contributor.author
Paschinger, Katharina
dc.date.accessioned
2019-02-06T16:00:11Z
dc.date.available
2019-01-18T04:21:25Z
dc.date.available
2019-02-06T16:00:11Z
dc.date.issued
2019-01-08
dc.identifier.issn
2041-1723
dc.identifier.other
10.1038/s41467-018-07948-7
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/317101
dc.identifier.doi
10.3929/ethz-b-000317101
dc.description.abstract
The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change. In a four-dimensional analysis involving HPLC, MALDI-TOF–MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity. We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures. While some modifications including LacdiNAc, chitobiose, α1,3-fucose and phosphorylcholine are familiar, anionic N-glycans have previously not been reported in nematodes. Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q. Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Highly modified and immunoactive N-glycans of the canine heartworm
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Nature Communications
ethz.journal.volume
10
en_US
ethz.journal.abbreviated
Nat Commun
ethz.pages.start
75
en_US
ethz.size
18 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-01-18T04:21:25Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-02-06T16:00:25Z
ethz.rosetta.lastUpdated
2020-02-15T16:57:41Z
ethz.rosetta.versionExported
true
ethz.COinS
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