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dc.contributor.author
Vonderheit, Andreas
dc.contributor.author
Helenius, Ari
dc.date.accessioned
2019-06-05T16:08:36Z
dc.date.available
2017-06-09T10:05:50Z
dc.date.available
2019-06-05T16:08:36Z
dc.date.issued
2005-06-21
dc.identifier.issn
1544-9173
dc.identifier.issn
1545-7885
dc.identifier.other
10.1371/journal.pbio.0030233
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/32091
dc.identifier.doi
10.3929/ethz-b-000032091
dc.description.abstract
Semliki forest virus (SFV) is internalized by clathrin-mediated endocytosis, and transported via early endosomes to late endosomes and lysosomes. The intracellular pathway taken by individual fluorescently labeled SFV particles was followed using immunofluorescence in untransfected cells, and by video-enhanced, triple-color fluorescence microscopy in live cells transfected with GFP- and RFP-tagged Rab5, Rab7, Rab4, and Arf1. The viruses progressed from Rab5-positive early endosomes to a population of early endosomes (about 10% of total) that contained both Rab5 and Rab7. SFV were sequestered in the Rab7 domains, and they were sorted away from the early endosomes when these domains detached as separate transport carriers devoid of Rab5, Rab4, EEA1, Arf1, and transferrin. The process was independent of Arf1 and the acidic pH in early endosomes. Nocodazole treatment showed that the release of transport carriers was assisted by microtubules. Expression of constitutively inactive Rab7T22N resulted in accumulation of SFV in early endosomes. We concluded that Rab7 is recruited to early endosomes, where it forms distinct domains that mediate cargo sorting as well as the formation of late-endosome-targeted transport vesicles.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.title
Rab7 Associates with Early Endosomes to Mediate Sorting and Transport of Semliki Forest Virus to Late Endosomes
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
ethz.journal.title
PLoS Biology
ethz.journal.volume
3
en_US
ethz.journal.issue
7
en_US
ethz.journal.abbreviated
PLoS biol.
ethz.pages.start
1225
en_US
ethz.pages.end
1238
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004606893
ethz.publication.place
Lawrence, KS
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
03495 - Helenius, Ari
en_US
ethz.leitzahl.certified
03495 - Helenius, Ari
ethz.date.deposited
2017-06-09T10:06:19Z
ethz.source
ECIT
ethz.identifier.importid
imp59364dcda06e151069
ethz.ecitpid
pub:52638
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T10:33:29Z
ethz.rosetta.lastUpdated
2019-06-05T16:08:46Z
ethz.rosetta.versionExported
true
ethz.COinS
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