Microfluidic multi-organ platform to study the effect of prodrugs on early embryonic development
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Assessing the toxicity of prodrugs still constitutes a major challenge in current cell-based assays. Here, we present a microfluidic platform to test the toxic effects of liver-activated prodrugs on early embryonic development. Embryoid bodies (EBs) and human liver microtissues (hLiMTs, InSphero AG) were combined in a hanging-drop network (HDN, ) which features an ideal environment for the formation and cultivation of 3D spherical microtissues. By tilting the device, gravity-driven flow through the network was induced and allowed for continuous compound-tissue interaction. As a proof of concept, the prodrug cyclophosphamide (CP) was investigated, the metabolites of which are known to be teratogenic. Show more
Book title22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectMicrofluidics; Hanging drops; 3D microtissues; Embryonic stem cell tests; Embryoid bodies
Organisational unit03684 - Hierlemann, Andreas / Hierlemann, Andreas
NotesExtended abstract. Conference lecture on November 14, 2018.
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