Show simple item record

dc.contributor.author
Rollins, Katherine A.
dc.contributor.author
Opitz, Lennart
dc.contributor.author
Arnold, Myrtha
dc.contributor.author
Simon, Eric
dc.contributor.author
Neubauer, Heike
dc.contributor.author
Wolfrum, Susanne
dc.date.accessioned
2019-02-12T16:03:03Z
dc.date.available
2019-02-01T04:49:48Z
dc.date.available
2019-02-12T16:03:03Z
dc.date.issued
2019-03
dc.identifier.other
10.1016/j.peptides.2019.01.001
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/322354
dc.identifier.doi
10.3929/ethz-b-000322354
dc.description.abstract
Altered GLP-1 secretion from L cells has been implicated in the development of type 2 diabetes mellitus and its resolution following bariatric surgery. However, changes in L cell gene expression, which may form the basis for altered functionality after high fat diet (HFD) or bariatric surgery, have either not been investigated or have given conflicting results. We developed a gcg-DTR-eGFP reporter mouse to isolate ileal and colonic L cells from HFD fed insulin resistant mice and mice showing improved glucose tolerance following vertical sleeve gastrectomy (VSG). Transcriptomic sequencing and identification of genes differentially expressed in response to HFD or VSG revealed small changes with HFD, primarily in immune related genes, but no regulation following VSG. In contrast, large differences were observed between ileal and colonic L cells due to the differential expression of genes involved in nutrient transport and metabolism, reflecting to some extent the differences in the surrounding epithelium. We showed that, in line with the gene expression data, colonic and ileal L cells exhibit differing GLP-1 responses to nutrients (glucose and the gly-sar dipeptide) and hormones (vasopressin). Thus, we hypothesise that colonic and ileal L cells have different physiological roles, with ileal L cells contributing more to postprandial glucose homeostasis by responding to dietary nutrients and colonic cells responding more to non-dietary stimulants.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
GLP-1
en_US
dc.subject
Enteroendocrine L cells
en_US
dc.subject
RNA sequencing
en_US
dc.subject
High fat diet
en_US
dc.subject
Vertical sleeve gastrectomy
en_US
dc.title
The L cell transcriptome is unaffected by vertical sleeve gastrectomy but highly dependent upon position within the gastrointestinal tract
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2019-01-17
ethz.journal.title
Peptides
ethz.journal.volume
113
en_US
ethz.pages.start
22
en_US
ethz.pages.end
34
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-02-01T04:49:54Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-02-12T16:03:13Z
ethz.rosetta.lastUpdated
2019-02-12T16:03:13Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=The%20L%20cell%20transcriptome%20is%20unaffected%20by%20vertical%20sleeve%20gastrectomy%20but%20highly%20dependent%20upon%20position%20within%20the%20gastrointestinal%20&rft.jtitle=Peptides&rft.date=2019-03&rft.volume=113&rft.spage=22&rft.epage=34&rft.au=Rollins,%20Katherine%20A.&Opitz,%20Lennart&Arnold,%20Myrtha&Simon,%20Eric&Neubauer,%20Heike&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record