Show simple item record

dc.contributor.author
Allen, Richard C.
dc.contributor.author
Angst, Daniel C.
dc.contributor.author
Hall, Alex R.
dc.date.accessioned
2019-02-22T16:58:05Z
dc.date.available
2019-02-20T05:48:19Z
dc.date.available
2019-02-22T16:58:05Z
dc.date.issued
2019-02
dc.identifier.issn
0099-2240
dc.identifier.issn
1098-5336
dc.identifier.other
10.1128/AEM.02111-18
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/326332
dc.identifier.doi
10.3929/ethz-b-000326332
dc.description.abstract
Bacterial pathogens that carry antibiotic resistance alleles sometimes pay a cost in the form of impaired growth in antibiotic-free conditions. This cost of resistance is expected to be a key parameter for understanding how resistance spreads and persists in pathogen populations. Analysis of individual resistance alleles from laboratory evolution and natural isolates has shown they are typically costly, but these costs are highly variable and influenced by genetic variation at other loci. It therefore remains unclear how strongly resistance is linked to impaired antibiotic-free growth in bacteria from natural and clinical scenarios, where resistance alleles are likely to coincide with other types of genetic variation. To investigate this, we measured the growth of 92 natural and clinical Escherichia coli isolates across three antibiotic-free environments. We then tested whether variation of antibiotic-free growth among isolates was predicted by their resistance to 10 antibiotics, while accounting for the phylogenetic structure of the data. We found that isolates with similar resistance profiles had similar antibiotic-free growth profiles, but it was not simply that higher average resistance was associated with impaired growth. Next, we used whole-genome sequences to identify antibiotic resistance genes and found that isolates carrying a greater number of resistance gene types grew relatively poorly in antibiotic-free conditions, even when the resistance genes they carried were different. This suggests that the resistance of bacterial pathogens is linked to growth costs in nature, but it is the total genetic burden and multivariate resistance phenotype that predict these costs, rather than individual alleles or mean resistance across antibiotics.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Microbiology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Escherichia coli
en_US
dc.subject
fitness
en_US
dc.subject
antibiotic resistance
en_US
dc.title
Resistance Gene Carriage Predicts Growth of Natural and Clinical Escherichia coli Isolates in the Absence of Antibiotics
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-02-06
ethz.journal.title
Applied and Environmental Microbiology
ethz.journal.volume
85
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
Appl. Environ. Microbiol
ethz.pages.start
e02111-18
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03584 - Bonhoeffer, Sebastian / Bonhoeffer, Sebastian
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03584 - Bonhoeffer, Sebastian / Bonhoeffer, Sebastian
ethz.date.deposited
2019-02-20T05:48:36Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-02-22T16:58:11Z
ethz.rosetta.lastUpdated
2025-02-13T18:43:19Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Resistance%20Gene%20Carriage%20Predicts%20Growth%20of%20Natural%20and%20Clinical%20Escherichia%20coli%20Isolates%20in%20the%20Absence%20of%20Antibiotics&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.date=2019-02&rft.volume=85&rft.issue=4&rft.spage=e02111-18&rft.issn=0099-2240&1098-5336&rft.au=Allen,%20Richard%20C.&Angst,%20Daniel%20C.&Hall,%20Alex%20R.&rft.genre=article&rft_id=info:doi/10.1128/AEM.02111-18&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record