Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain
Düring, Daniel N.
Rocha, Mariana D.
Hahnloser, Richard H.R.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
Expansion microscopy and light sheet imaging (ExLSM) provide a viable alternative to existing tissue clearing and large volume imaging approaches. The analysis of intact volumes of brain tissue presents a distinct challenge in neuroscience. Recent advances in tissue clearing and light sheet microscopy have re-addressed this challenge and blossomed into a plethora of protocols with diverse advantages and disadvantages. While refractive index matching achieves near perfect transparency and allows for imaging at large depths, the resolution of cleared brains is usually limited to the micrometer range. Moreover, the often long and harsh tissue clearing protocols hinder preservation of native fluorescence and antigenicity. Here we image large expanded brain volumes of zebra finch brain tissue in commercially available light sheet microscopes. Our expansion light sheet microscopy (ExLSM) approach presents a viable alternative to many clearing and imaging methods because it improves on tissue processing times, fluorophore compatibility, and image resolution. Show more
Journal / seriesFrontiers in Neuroanatomy
Pages / Article No.
PublisherFrontiers Research Foundation
SubjectExpansion microscopy; Tissue clearing; Songbird; Spine morphology; Light sheet microscopy; Large volume imaging; Super resolution microscopy
Organisational unit03774 - Hahnloser, Richard H.R. / Hahnloser, Richard H.R.
750055 - Development of synaptic networks in songbirds (EC)
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