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dc.contributor.author
Bracher, Susanne
dc.contributor.author
Hilger, Daniel
dc.contributor.author
Guérin, Kamila
dc.contributor.author
Polyhach, Yevhen
dc.contributor.author
Jeschke, Gunnar
dc.contributor.author
Krafczyk, Ralph
dc.contributor.author
Giacomelli, Giacomo
dc.contributor.author
Jung, Heinrich
dc.date.accessioned
2019-03-19T09:09:28Z
dc.date.available
2019-03-16T04:14:13Z
dc.date.available
2019-03-19T09:09:28Z
dc.date.issued
2019
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/s41598-019-40516-7
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/331768
dc.identifier.doi
10.3929/ethz-b-000331768
dc.description.abstract
Secondary transporters exist as monomers, dimers or higher state oligomers. The significance of the oligomeric state is only partially understood. Here, the significance of the trimeric state of the L-carnitine/γ-butyrobetaine antiporter CaiT of Escherichia coli was investigated. Amino acids important for trimer stability were identified and experimentally verified. Among others, CaiT-D288A and -D288R proved to be mostly monomeric in detergent solution and after reconstitution into proteoliposomes, as shown by blue native gel electrophoresis, gel filtration, and determination of intermolecular distances. CaiT-D288A was fully functional with kinetic parameters similar to the trimeric wild-type. Significant differences in amount and stability in the cell membrane between monomeric and trimeric CaiT were not observed. Contrary to trimeric CaiT, addition of substrate had no or only a minor effect on the tryptophan fluorescence of monomeric CaiT. The results suggest that physical contacts between protomers are important for the substrate-induced changes in protein fluorescence and the underlying conformational alterations.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Comparison of the functional properties of trimeric and monomeric CaiT of Escherichia coli
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-03-07
ethz.journal.title
Scientific Reports
ethz.journal.volume
9
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
3787
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03810 - Jeschke, Gunnar / Jeschke, Gunnar
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03810 - Jeschke, Gunnar / Jeschke, Gunnar
ethz.date.deposited
2019-03-16T04:14:13Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-03-19T09:09:43Z
ethz.rosetta.lastUpdated
2021-02-15T04:02:05Z
ethz.rosetta.versionExported
true
ethz.COinS
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