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dc.contributor.author
Penzel, Susanne
dc.contributor.author
Oss, Andres
dc.contributor.author
Org, Mai-Liis
dc.contributor.author
Samoson, Ago
dc.contributor.author
Böckmann, Anja
dc.contributor.author
Ernst, Matthias
dc.contributor.author
Meier, Beat H.
dc.date.accessioned
2019-04-08T07:21:18Z
dc.date.available
2019-04-06T01:36:02Z
dc.date.available
2019-04-08T07:21:18Z
dc.date.issued
2019-02
dc.identifier.issn
0925-2738
dc.identifier.issn
1573-5001
dc.identifier.other
10.1007/s10858-018-0219-9
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/336604
dc.identifier.doi
10.3929/ethz-b-000336604
dc.description.abstract
We report linewidth and proton T1, T1ρ and T2′ relaxation data of the model protein ubiquitin acquired at MAS frequencies up to 126 kHz. We find a predominantly linear improvement in linewidths and coherence decay times of protons with increasing spinning frequency in the range from 93 to 126 kHz. We further attempt to gain insight into the different contributions to the linewidth at fast MAS using site-specific analysis of proton relaxation parameters and present bulk relaxation times as a function of the MAS frequency. For microcrystalline fully-protonated ubiquitin, inhomogeneous contributions are only a minor part of the proton linewidth, and at 126 kHz MAS coherent effects are still dominating. We furthermore present site-specific proton relaxation rate constants during a spinlock at 126 kHz MAS, as well as MAS-dependent bulk T1ρ (1HN).
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Fast MAS
en_US
dc.subject
Proton detection
en_US
dc.subject
Solid-state NMR
en_US
dc.title
Spinning faster: protein NMR at MAS frequencies up to 126 kHz
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-01-24
ethz.journal.title
Journal of Biomolecular NMR
ethz.journal.volume
73
en_US
ethz.journal.issue
1-2
en_US
ethz.journal.abbreviated
J. biomol. NMR
ethz.pages.start
19
en_US
ethz.pages.end
29
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
NMR studies in the Solid State
en_US
ethz.grant
NMR studies in the Solid State
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Berlin
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03496 - Meier, Beat H. / Meier, Beat H.
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03810 - Jeschke, Gunnar / Jeschke, Gunnar::08829 - Ernst, Matthias (Tit.-Prof.)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03496 - Meier, Beat H. / Meier, Beat H.
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03810 - Jeschke, Gunnar / Jeschke, Gunnar::08829 - Ernst, Matthias (Tit.-Prof.)
ethz.grant.agreementno
159707
ethz.grant.agreementno
178792
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Mathematik, Natur- und Ingenieurwissenschaften (Abteilung II)
ethz.grant.program
Projektförderung in Mathematik, Natur- und Ingenieurwissenschaften (Abteilung II)
ethz.date.deposited
2019-04-06T01:36:24Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-04-08T07:21:39Z
ethz.rosetta.lastUpdated
2021-02-15T04:17:12Z
ethz.rosetta.versionExported
true
ethz.COinS
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