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dc.contributor.author
Marchais, Antonin
dc.contributor.author
Chevalier, Clément
dc.contributor.author
Voinnet, Olivier
dc.date.accessioned
2020-11-02T10:45:17Z
dc.date.available
2019-06-05T08:07:31Z
dc.date.available
2019-06-05T08:44:44Z
dc.date.available
2019-08-30T12:35:20Z
dc.date.available
2020-11-02T10:45:17Z
dc.date.issued
2019-09
dc.identifier.issn
1355-8382
dc.identifier.issn
1469-9001
dc.identifier.other
10.1261/rna.069294.118
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/345521
dc.identifier.doi
10.3929/ethz-b-000345521
dc.description.abstract
In a reductionist perspective, plant silencing small (s)RNAs are often classified as mediating nuclear transcriptional gene silencing (TGS) or cytosolic posttranscriptional gene silencing (PTGS). Among the PTGS diagnostics is the association of AGOs and their sRNA cargos with the translation apparatus. In Arabidopsis, this is observed for AGO1 loaded with micro(mi)RNAs and, accordingly, translational-repression (TR) is one layer of plant miRNA action. Using AGO1:miRNA-mediated TR as a paradigm, we explored, with two unrelated polysome-isolation methods, which, among the ten Arabidopsis AGOs and numerous sRNA classes, interact with translation. We found that representatives of all three AGO-clades associate with polysomes, including the TGS-effector AGO4 and stereotypical 24-nt sRNAs that normally mediate TGS of transposons/repeats. Strikingly, approximately half of these annotated 24-nt siRNAs displayed unique matches in coding regions/introns of genes, and in pseudogenes, but not in transposons/repeats commonly found in their vicinity. Protein-coding gene-derived 24-nt sRNAs correlate with gene-body methylation. Those derived from pseudogenes belong to two main clusters defined by their parental-gene expression patterns, and are vastly enriched in AGO5, itself found on polysomes. Based on their tight expression pattern in developing and mature siliques, their biogenesis, and genomic/epigenomic features of their loci-of-origin, we discuss potential roles for these hitherto unknown polysome-enriched, pseudogene-derived siRNAs.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Cold Spring Harbor Laboratory Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.subject
Eukaryota
en_US
dc.subject
Plants
en_US
dc.subject
Polysome
en_US
dc.subject
small RNAs
en_US
dc.title
Extensive profiling in Arabidopsis reveals abundant polysome-associated 24-nt small RNAs including AGO5-dependent pseudogene-derived siRNAs
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
dc.date.published
2019-05-28
ethz.journal.title
RNA
ethz.journal.volume
25
en_US
ethz.journal.issue
9
en_US
ethz.pages.start
1098
en_US
ethz.pages.end
1117
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
High resolution and chemical genetic approaches to RNA silencing mechanisms
en_US
ethz.identifier.wos
ethz.publication.place
Cold Spring Harbor, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03876 - Voinnet, Olivier / Voinnet, Olivier
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03876 - Voinnet, Olivier / Voinnet, Olivier
en_US
ethz.grant.agreementno
323071
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
FP7
ethz.date.deposited
2019-06-05T08:07:41Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.date.embargoend
2020-10-15
ethz.rosetta.installDate
2019-08-30T12:35:40Z
ethz.rosetta.lastUpdated
2021-02-15T19:39:33Z
ethz.rosetta.versionExported
true
ethz.COinS
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