Show simple item record

dc.contributor.author
Mulas, Carla
dc.contributor.author
Kalkan, Tüzer
dc.contributor.author
von Meyenn, Ferdinand
dc.contributor.author
Leitch, Harry G.
dc.contributor.author
Nichols, Jennifer
dc.contributor.author
Smith, Austin
dc.date.accessioned
2019-06-11T15:22:09Z
dc.date.available
2019-06-07T09:23:28Z
dc.date.available
2019-06-11T15:22:09Z
dc.date.issued
2019-03-26
dc.identifier.issn
0950-1991
dc.identifier.issn
1477-9129
dc.identifier.other
10.1242/dev.173146
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/346261
dc.identifier.doi
10.3929/ethz-b-000346261
dc.description.abstract
The power of mouse embryonic stem (ES) cells to colonise the developing embryo has revolutionised mammalian developmental genetics and stem cell research. This power is vulnerable, however, to the cell culture environment, deficiencies in which can lead to cellular heterogeneity, adaptive phenotypes, epigenetic aberrations and genetic abnormalities. Here, we provide detailed methodologies for derivation, propagation, genetic modification and primary differentiation of ES cells in 2i or 2i+LIF media without serum or undefined serum substitutes. Implemented diligently, these procedures minimise variability and deviation, thereby improving the efficiency, reproducibility and biological validity of ES cell experimentation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
The Company of Biologists
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Differentiation
en_US
dc.subject
Embryonic stem cells
en_US
dc.subject
Pluripotency
en_US
dc.subject
Self-renewal
en_US
dc.title
Defined conditions for propagation and manipulation of mouse embryonic stem cells
en_US
dc.type
Journal Article
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Development
ethz.journal.volume
146
en_US
ethz.journal.abbreviated
Development (Camb.)
ethz.pages.start
dev173146
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
Cambridge
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09658 - von Meyenn, Ferdinand / von Meyenn, Ferdinand
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09658 - von Meyenn, Ferdinand / von Meyenn, Ferdinand
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09658 - von Meyenn, Ferdinand / von Meyenn, Ferdinand
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09658 - von Meyenn, Ferdinand / von Meyenn, Ferdinand
ethz.identifier.orcidWorkCode
55740868
ethz.date.deposited
2019-06-07T09:23:35Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-06-11T15:22:24Z
ethz.rosetta.lastUpdated
2020-02-15T19:31:11Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Defined%20conditions%20for%20propagation%20and%20manipulation%20of%20mouse%20embryonic%20stem%20cells&rft.jtitle=Development&rft.date=2019-03-26&rft.volume=146&rft.spage=dev173146&rft.issn=0950-1991&1477-9129&rft.au=Mulas,%20Carla&Kalkan,%20T%C3%BCzer&von%20Meyenn,%20Ferdinand&Leitch,%20Harry%20G.&Nichols,%20Jennifer&rft.genre=article&article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record