Show simple item record

dc.contributor.author
Peng, Keli
dc.contributor.author
Li, Xu
dc.contributor.author
Wu, Congyu
dc.contributor.author
Wang, Yuna
dc.contributor.author
Yu, Jian
dc.contributor.author
Zhang, Jinxing
dc.contributor.author
Gao, Qian
dc.contributor.author
Zhang, Wenhao
dc.contributor.author
Zhang, Qian
dc.contributor.author
Fan, Yong
dc.contributor.author
Yu, Yang
dc.contributor.author
Shuai, Ling
dc.date.accessioned
2019-06-12T06:38:26Z
dc.date.available
2019-06-08T12:14:42Z
dc.date.available
2019-06-12T06:38:26Z
dc.date.issued
2019-01-25
dc.identifier.other
10.1016/j.isci.2018.12.014
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/346438
dc.identifier.doi
10.3929/ethz-b-000346438
dc.description.abstract
Owing to their single genome, haploid cells are powerful to uncover unknown genes by performing genetic screening in mammals. However, no haploid cell line from an extraembryonic lineage has been achieved yet, which limits the application of haploid cells in placental genetic screening. Here, we show that overexpression of Cdx2 can convert haploid embryonic stem cells to trophoblast stem cells (TSCs). p53 deletion reduces diploidization during the conversion and guarantees the generation of haploid-induced TSCs (haiTSCs). haiTSCs not only share the same molecular characterization with trophoderm-derived TSCs but also possess multipotency to placental lineages in various procedures. In addition, haiTSCs can maintain haploidy in the long term, assisted by periodic sorting and with reliance on FGF4 and heparin. Finally, we perform piggyBac-mediated high-throughput mutation in haiTSCs and use them in trophoblast lineage genetic screening. Deep sequencing analysis and validation experiments prove that Htra1 is a blocker for spongiotrophoblast specification.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Cell Biology
en_US
dc.subject
Stem Cells Research
en_US
dc.subject
Genomics
en_US
dc.title
Derivation of Haploid Trophoblast Stem Cells via Conversion In Vitro
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2019-01-08
ethz.journal.title
iScience
ethz.journal.volume
11
en_US
ethz.pages.start
508
en_US
ethz.pages.end
518
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-06-08T12:14:45Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-06-12T06:38:55Z
ethz.rosetta.lastUpdated
2020-02-15T19:31:26Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Derivation%20of%20Haploid%20Trophoblast%20Stem%20Cells%20via%20Conversion%20In%20Vitro&rft.jtitle=iScience&rft.date=2019-01-25&rft.volume=11&rft.spage=508&rft.epage=518&rft.au=Peng,%20Keli&Li,%20Xu&Wu,%20Congyu&Wang,%20Yuna&Yu,%20Jian&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record