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dc.contributor.author
Ceresini, Paulo C.
dc.contributor.author
Lacerda Soares Petrarolha Silva, Cristina
dc.contributor.author
Missio, Robson F.
dc.contributor.author
Costa Souza, Elaine
dc.contributor.author
Fischer, Carlos N.
dc.contributor.author
Guillherme, Ivan R.
dc.contributor.author
Gregorio, Ivo
dc.contributor.author
Tajara da Silva, Eloiza H.
dc.contributor.author
Barreto Cicarelli, Regina M.
dc.contributor.author
Alves da Silva, Marco T.
dc.contributor.author
Garcia, José F.
dc.contributor.author
Avelar, Gustavo A.
dc.contributor.author
Porto Neto, Laercio R.
dc.contributor.author
Marçon, André R.
dc.contributor.author
Bacci Junior, Maurício
dc.contributor.author
Marini, Danyelle C.
dc.date.accessioned
2019-10-16T12:35:45Z
dc.date.available
2017-06-09T10:58:58Z
dc.date.available
2018-03-09T14:55:13Z
dc.date.available
2019-10-16T12:35:45Z
dc.date.issued
2005
dc.identifier.issn
1415-4757
dc.identifier.issn
1678-4685
dc.identifier.other
10.1590/S1415-47572005000400014
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/34680
dc.identifier.doi
10.3929/ethz-b-000034680
dc.description.abstract
The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most frequent (35.15%) followed by the trimeric CCG/CGG (12.81%). From a random sample of 1,217 sequences, 343 microsatellites in 265 SSR-containing sequences were identified. Approximately 48% of these ESTs containing microsatellites were homologous to proteins with known biological function. Most of the microsatellites detected in Eucalyptus ESTs were positioned at either the 5 or 3 end. Our next priority involves the design of flanking primers for codominant SSR loci, which could lead to the development of a set of microsatellite-based markers suitable for marker-assisted Eucalyptus breeding programs.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
SBG
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.subject
Bioinformatics tool
en_US
dc.subject
Genetic markers
en_US
dc.subject
Gene ontology
en_US
dc.subject
Small sequence repeats
en_US
dc.subject
SSR
en_US
dc.title
Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
ethz.journal.title
Genetics and Molecular Biology
ethz.journal.volume
28
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
Genet. Mol. Biol.
ethz.pages.start
589
en_US
ethz.pages.end
600
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
001979632
ethz.publication.place
Ribeirão Preto
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
ethz.date.deposited
2017-06-09T10:59:14Z
ethz.source
ECIT
ethz.identifier.importid
imp59364e03e351e57600
ethz.ecitpid
pub:55795
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-31T15:27:11Z
ethz.rosetta.lastUpdated
2019-10-16T12:35:57Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Satellyptus:%20analysis%20and%20database%20of%20microsatellites%20from%20ESTs%20of%20Eucalyptus&rft.jtitle=Genetics%20and%20Molecular%20Biology&rft.date=2005&rft.volume=28&rft.issue=3&rft.spage=589&rft.epage=600&rft.issn=1415-4757&1678-4685&rft.au=Ceresini,%20Paulo%20C.&Lacerda%20Soares%20Petrarolha%20Silva,%20Cristina&Missio,%20Robson%20F.&Costa%20Souza,%20Elaine&Fischer,%20Carlos%20N.&rft.genre=article&
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