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dc.contributor.author
Brugger, Silvio D.
dc.contributor.author
Kraemer, Julia G.
dc.contributor.author
Qi, Weihong
dc.contributor.author
Bomar, Lindsey
dc.contributor.author
Oppliger, Anne
dc.contributor.author
Hilty, Markus
dc.date.accessioned
2019-07-02T07:57:05Z
dc.date.available
2019-07-02T02:09:30Z
dc.date.available
2019-07-02T07:57:05Z
dc.date.issued
2019-06-19
dc.identifier.issn
1664-8021
dc.identifier.other
10.3389/fgene.2019.00555
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/350771
dc.identifier.doi
10.3929/ethz-b-000350771
dc.description.abstract
Acute bacterial otitis media is usually caused by otopathogens ascending to the middle ear from the nasopharynx (NP). However, it is unknown if the nasopharyngeal microbiota of children with acute otitis media (AOM) can serve as an age-dependent or independent proxy for the microbial communities of the middle ear fluid (MEF) as there is a lack of 16S rRNA amplicon sequencing studies simultaneously analyzing the microbial communities of the two sites. Within this study, we performed 16S rRNA next generation sequencing on a total of 286 nasopharyngeal swabs (NPSs) collected between 2004 and 2013 within a Swiss national AOM surveillance program from children (0–6 years) with AOM. In addition, 42/286 children had spontaneous tympanic membrane perforation and, therefore, those MEF could also be analyzed. We found that alpha [Richness, Shannon diversity index (SDI) and Evenness] and beta diversity measurements of the nasopharyngeal bacterial microbiota showed a clear dependency of the increasing age of the children. In more detail, bacterial richness and personalized profiles (measured by beta dispersion) were higher and more frequent in older children, respectively. Dissimilarity values based on the binary distance matrix of the microbiota patterns of the NP and the MEF also correlated with increasing age. In general, positive (PPV) and negative predictive values (NPV) of the most abundant operational taxonomic units (OTUs) in the NP were moderately and well predictive for their presence in the MEF, respectively. This data is crucial to better understand polymicrobial infections and therefore AOM pathogenesis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Research Foundation
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
nasopharyngeal microbiota
en_US
dc.subject
bacterial families
en_US
dc.subject
toddlers
en_US
dc.subject
acute otitis media
en_US
dc.subject
middle ear fluid
en_US
dc.subject
age
en_US
dc.title
Age-Dependent Dissimilarity of the Nasopharyngeal and Middle Ear Microbiota in Children With Acute Otitis Media
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-06-19
ethz.journal.title
Frontiers in Genetics
ethz.journal.volume
10
en_US
ethz.journal.abbreviated
Front. genet.
ethz.pages.start
555
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.date.deposited
2019-07-02T02:09:34Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-07-02T07:57:17Z
ethz.rosetta.lastUpdated
2020-02-15T19:59:58Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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