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dc.contributor.author
Gressmann, Helga
dc.contributor.author
Linz, Bodo
dc.contributor.author
Ghai, Rohit
dc.contributor.author
Pleissner, Klaus-Peter
dc.contributor.author
Schlapbach, Ralph
dc.contributor.author
Yamaoka, Yoshio
dc.contributor.author
Kraft, Christian
dc.contributor.author
Suerbaum, Sebastian
dc.contributor.author
Meyer, Thomas F.
dc.contributor.author
Achtman, Mark
dc.date.accessioned
2018-10-31T14:23:05Z
dc.date.available
2017-06-09T11:06:24Z
dc.date.available
2018-10-31T14:23:05Z
dc.date.issued
2005-10-07
dc.identifier.issn
1553-7390
dc.identifier.issn
1553-7404
dc.identifier.other
10.1371/journal.pgen.0010043
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/35077
dc.identifier.doi
10.3929/ethz-b-000035077
dc.description.abstract
Sequence diversity and gene content distinguish most isolates of Helicobacter pylori. Even greater sequence differences differentiate distinct populations of H. pylori from different continents, but it was not clear whether these populations also differ in gene content. To address this question, we tested 56 globally representative strains of H. pylori and four strains of Helicobacter acinonychis with whole genome microarrays. Of the weighted average of 1,531 genes present in the two sequenced genomes, 25% are absent in at least one strain of H. pylori and 21% were absent or variable in H. acinonychis. We extrapolate that the core genome present in all isolates of H. pylori contains 1,111 genes. Variable genes tend to be small and possess unusual GC content; many of them have probably been imported by horizontal gene transfer. Phylogenetic trees based on the microarray data differ from those based on sequences of seven genes from the core genome. These discrepancies are due to homoplasies resulting from independent gene loss by deletion or recombination in multiple strains, which distort phylogenetic patterns. The patterns of these discrepancies versus population structure allow a reconstruction of the timing of the acquisition of variable genes within this species. Variable genes that are located within the cag pathogenicity island were apparently first acquired en bloc after speciation. In contrast, most other variable genes are of unknown function or encode restriction/modification enzymes, transposases, or outer membrane proteins. These seem to have been acquired prior to speciation of H. pylori and were subsequently lost by convergent evolution within individual strains. Thus, the use of microarrays can reveal patterns of gene gain or loss when examined within a phylogenetic context that is based on sequences of core genes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science (PLoS)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Gain and loss of multiple genes during the evolution of Helicobacter pylori
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS Genetics
ethz.journal.volume
1
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
PLoS Genet
ethz.pages.start
e43
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
005410305
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::08828 - Schlapbach, Ralph (Tit.-Prof.)
en_US
ethz.date.deposited
2017-06-09T11:06:41Z
ethz.source
ECIT
ethz.identifier.importid
imp59364e0a8127c45057
ethz.ecitpid
pub:56310
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T17:00:10Z
ethz.rosetta.lastUpdated
2018-10-31T14:23:26Z
ethz.rosetta.versionExported
true
ethz.COinS
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