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dc.contributor.author
Lilja, Elin E.
dc.contributor.author
Johnson, David R.
dc.date.accessioned
2019-07-08T08:36:46Z
dc.date.available
2019-07-04T02:19:12Z
dc.date.available
2019-07-08T08:36:46Z
dc.date.issued
2019
dc.identifier.issn
1471-2148
dc.identifier.other
10.1186/s12862-019-1458-4
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/351266
dc.identifier.doi
10.3929/ethz-b-000351266
dc.description.abstract
Background: Substrate cross-feeding occurs when one organism partially consumes a primary substrate into one or more metabolites while other organisms then consume the metabolites. While pervasive within microbial communities, our knowledge about the effects of substrate cross-feeding on microbial evolution remains limited.To address this knowledge gap, we experimentally evolved isogenic nitrite (NO2−) cross-feeding microbial strains together for 700 generations, identified genetic changes that were acquired over the evolution experiment, and compared the results with an isogenic completely denitrifying strain that was evolved alone for 700 generations.We further investigated how the magnitude of interdependence between the nitrite cross-feeding strains affects the main outcomes. Our main objective was to quantify how substrate cross-feeding and the magnitude of interdependence affect the speed and trajectory of molecular evolution. Results:We found that each nitrite (NO2−) cross-feeding strain acquired fewer genetic changes than did the completely denitrifying strain. In contrast, pairs of nitrite cross-feeding strains together acquired more genetic changes than did the completely denitrifying strain. Moreover, nitrite cross-feeding promoted population diversification, as pairs of nitrite cross-feeding strains acquired a more varied set of genetic changes than did the completely denitrifying strain. These outcomes likely occurred because nitrite cross-feeding enabled the co-existence of two distinct microbial strains, thus increasing the amount of genetic variation for selection to act upon.Finally, the nitrite cross-feeding strains acquired different types of genetic changes than did the completely denitrifying strain, indicating that nitrite cross-feeding modulates the trajectory of molecular evolution. Conclusions:Our results demonstrate that substrate cross-feeding can affect both the speed and trajectory of molecular evolution within microbial populations. Substrate cross-feeding can therefore have potentially important effects on the life histories of microorganisms.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Experimental evolution
en_US
dc.subject
Cross-feeding
en_US
dc.subject
Microbial interactions
en_US
dc.subject
Denitrification
en_US
dc.subject
Molecular evolution
en_US
dc.subject
Mutualism
en_US
dc.title
Substrate cross-feeding affects the speed and trajectory of molecular evolution within a synthetic microbial assemblage
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-06-20
ethz.journal.title
BMC Evolutionary Biology
ethz.journal.volume
19
en_US
ethz.journal.issue
129
en_US
ethz.journal.abbreviated
BMC evol. biol.
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.date.deposited
2019-07-04T02:19:15Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-07-08T08:36:57Z
ethz.rosetta.lastUpdated
2019-07-08T08:36:57Z
ethz.rosetta.versionExported
true
ethz.COinS
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