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dc.contributor.author
Gottschlich, Lisa
dc.contributor.author
Geiser, Petra
dc.contributor.author
Bortfeld-Miller, Miriam
dc.contributor.author
Field, Christopher M.
dc.contributor.author
Vorholt, Julia A.
dc.date.accessioned
2019-07-08T09:31:54Z
dc.date.available
2019-07-07T02:37:11Z
dc.date.available
2019-07-08T09:31:54Z
dc.date.issued
2019
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/s41598-019-45788-7
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/351713
dc.identifier.doi
10.3929/ethz-b-000351713
dc.description.abstract
The general stress response (GSR) represents an important trait to survive in the environment by leading to multiple stress resistance. In alphaproteobacteria, the GSR is under the transcriptional control of the alternative sigma factor EcfG. Here we performed transcriptome analyses to investigate the genes controlled by EcfG of Sphingomonas melonis Fr1 and the plasticity of this regulation under stress conditions. We found that EcfG regulates genes for proteins that are typically associated with stress responses. Moreover, EcfG controls regulatory proteins, which likely fine-tune the GSR. Among these, we identified a novel negative GSR feedback regulator, termed NepR2, on the basis of gene reporter assays, phenotypic analyses, and biochemical assays. Transcriptional profiling of signaling components upstream of EcfG under complex stress conditions showed an overall congruence with EcfG-regulated genes. Interestingly however, we found that the GSR is transcriptionally linked to the regulation of motility and biofilm formation via the single domain response regulator SdrG and GSR-activating histidine kinases. Altogether, our findings indicate that the GSR in S. melonis Fr1 underlies a complex regulation to optimize resource allocation and resilience in stressful and changing environments.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Complex general stress response regulation in Sphingomonas melonis Fr1 revealed by transcriptional analyses
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-06-28
ethz.journal.title
Scientific Reports
ethz.journal.volume
9
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
9404
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Investigation of the alphaproteobacterial general stress response in the context of the phyllosphere environment
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.grant.agreementno
152835
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Bonus of Excellence
ethz.date.deposited
2019-07-07T02:37:17Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-07-08T09:32:05Z
ethz.rosetta.lastUpdated
2019-07-08T09:32:05Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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