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dc.contributor.author
Weiss, Gregor L.
dc.contributor.author
Kieninger, Ann-Katrin
dc.contributor.author
Maldener, Iris
dc.contributor.author
Forchhammer, Karl
dc.contributor.author
Pilhofer, Martin
dc.date.accessioned
2019-07-16T15:30:52Z
dc.date.available
2019-07-13T02:29:38Z
dc.date.available
2019-07-16T15:30:52Z
dc.date.issued
2019-07-11
dc.identifier.issn
0092-8674
dc.identifier.issn
1097-4172
dc.identifier.other
10.1016/j.cell.2019.05.055
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/353082
dc.identifier.doi
10.3929/ethz-b-000353082
dc.description.abstract
Multicellular lifestyle requires cell-cell connections. In multicellular cyanobacteria, septal junctions enable molecular exchange between sister cells and are required for cellular differentiation. The structure of septal junctions is poorly understood, and it is unknown whether they are capable of controlling intercellular communication. Here, we resolved the in situ architecture of septal junctions by electron cryotomography of cryo-focused ion beam-milled cyanobacterial filaments. Septal junctions consisted of a tube traversing the septal peptidoglycan. Each tube end comprised a FraD-containing plug, which was covered by a cytoplasmic cap. Fluorescence recovery after photobleaching showed that intercellular communication was blocked upon stress. Gating was accompanied by a reversible conformational change of the septal junction cap. We provide the mechanistic framework for a cell junction that predates eukaryotic gap junctions by a billion years. The conservation of a gated dynamic mechanism across different domains of life emphasizes the importance of controlling molecular exchange in multicellular organisms.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Cell Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Multicellularity
en_US
dc.subject
cell-cell connections
en_US
dc.subject
membrane trafficking
en_US
dc.subject
septal junctions
en_US
dc.subject
cyanobacteria
en_US
dc.subject
electron cryotomography
en_US
dc.subject
subtomogram averaging
en_US
dc.subject
fluorescence recovery after photobleaching
en_US
dc.title
Structure and Function of a Bacterial Gap Junction Analog
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2019-07-11
ethz.journal.title
Cell
ethz.journal.volume
178
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
Cell
ethz.pages.start
374
en_US
ethz.pages.end
384.e15
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Structure, function, and evolution of bacterial contractile injection systems
en_US
ethz.grant
Multiscale model of bacterial cell-cell interactions
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Cambridge, MA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::09463 - Pilhofer, Martin / Pilhofer, Martin
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::09463 - Pilhofer, Martin / Pilhofer, Martin
ethz.grant.agreementno
179255
ethz.grant.agreementno
679209
ethz.grant.fundername
SNF
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
H2020
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2019-07-13T02:29:45Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-07-16T15:31:09Z
ethz.rosetta.lastUpdated
2021-02-15T05:17:03Z
ethz.rosetta.versionExported
true
ethz.COinS
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