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dc.contributor.author
Begheyn, Rachel F.
dc.contributor.author
Yates, Steven A.
dc.contributor.author
Sykes, Timothy
dc.contributor.author
Studer, Bruno
dc.date.accessioned
2019-07-24T06:56:24Z
dc.date.available
2019-07-24T06:56:24Z
dc.date.issued
2018-06-01
dc.identifier.issn
2160-1836
dc.identifier.other
10.1534/g3.117.300550
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/354800
dc.identifier.doi
10.3929/ethz-b-000265925
dc.description.abstract
Immature pollen can be induced to switch developmental pathways from gametogenesis to embryogenesis and subsequently regenerate into homozygous, diploid plants. Such androgenic production of doubled haploids is particularly useful for species where inbreeding is hampered by effective self-incompatibility systems. Therefore, increasing the generally low androgenic capacity of perennial ryegrass (Lolium perenne L.) germplasm would enable the efficient production of homozygous plant material, so that a more effective exploitation of heterosis through hybrid breeding schemes can be realized. Here, we present the results of a genome-wide association study in a heterozygous, multiparental population of perennial ryegrass (n = 391) segregating for androgenic capacity. Genotyping-by-sequencing was used to interrogate gene- dense genomic regions and revealed over 1,100 polymorphic sites. Between one and 10 quantitative trait loci (QTL) were identified for anther response, embryo and total plant production, green and albino plant production and regeneration. Most traits were under polygenic control, although a major QTL on linkage group 5 was associated with green plant regeneration. Distinct genetic factors seem to affect green and albino plant recovery. Two intriguing candidate genes, encoding chromatin binding domains of the developmental phase transition regulator, Polycomb Repressive Complex 2, were identified. Our results shed the first light on the molecular mechanisms behind perennial ryegrass microspore embryogenesis and enable marker-assisted introgression of androgenic capacity into recalcitrant germplasm of this forage crop of global significance.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Genetics Society of America
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Anther culture (AC)
en_US
dc.subject
Doubled haploid (DH)
en_US
dc.subject
Perennial ryegrass (Lolium perenne L.)
en_US
dc.subject
Genome-wide association studies (GWAS)
en_US
dc.subject
Microspore embryogenesis (ME)
en_US
dc.subject
Multipareental populations
en_US
dc.title
Genetic Loci Governing Androgenic Capacity in Perennial Ryegrass (Lolium perenne L.)
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2018-05-31
ethz.journal.title
G3
ethz.journal.volume
8
en_US
ethz.journal.issue
6
en_US
ethz.journal.abbreviated
G3 (Bethesda Md.)
ethz.pages.start
1897
en_US
ethz.pages.end
1908
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
FUNCTIONAL GENOMICS OF GRASS REPRODUCTIVE TRAITS
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.pubmed
29626084
ethz.publication.place
Bethesda, MD
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.grant.agreementno
138983
ethz.grant.agreementno
138983
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
SNF-Förderungsprofessuren Stufe 2
ethz.grant.program
SNF-Förderungsprofessuren Stufe 2
ethz.date.deposited
2018-05-24T14:42:23Z
ethz.source
SCOPUS
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-07-24T06:56:31Z
ethz.rosetta.lastUpdated
2019-07-24T06:56:31Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/353903
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/265925
ethz.COinS
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