Yates, StevenShow all
- Other Conference Item
Rights / licenseIn Copyright - Non-Commercial Use Permitted
Grasses of the genera Lolium and Festuca are the main feed sources for a sustainable livestock production due to their high palatability and biomass production. Since decades, their importance for the agriculture of temperate regions led to the development of new varieties through traditional breeding programs. However, newer crop improvement methods such as genomic selection could benefit from a high quality reference genome assembly. In the past, attempts in delivering such a dataset have struggled due to the complexity of the genome and the high heterozygosity of individual genotypes. We sequenced an individual of the L. multiflorum (Italian ryegrass) cv. Rabiosa, producing a highly contiguous (N50 of 3 Mb) and complete assembly (97% of the BUSCO gene models). Due to the high heterozygosity of the line, the assembly (4.5 Gb) resulted to be as large as the diploid genome, and presented the sequence of both alleles in separate scaffolds. About ~70,000 gene models were identified, and the repeat content approached 80%. The comparison of a representative allelic region showed an extensive amount of intergenic sequence variation, supporting the high dynamicity of grass genomes. Compared to the available Lolium genome assemblies, the Rabiosa assembly improves contiguity by >40-fold, contains both haplotypes of the diploid parent, and assigns most of the sequence to chromosomes. The availability of a complete and highly-contiguous genome assembly of Italian ryegrass paves the road to the exploitation of the forage crops genetic resources by means of modern genomic platforms. Show more
External linksSearch via SFX
Pages / Article No.
Organisational unit03969 - Studer, Bruno / Studer, Bruno
MoreShow all metadata