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dc.contributor.author
Copetti, Dario
dc.contributor.author
Kuon, Joel‑Elias
dc.contributor.author
Yates, Steven
dc.contributor.author
Kölliker, Roland
dc.contributor.author
Studer, Bruno
dc.date.accessioned
2019-07-29T13:19:33Z
dc.date.available
2019-07-29T11:42:54Z
dc.date.available
2019-07-29T13:06:55Z
dc.date.available
2019-07-29T13:19:33Z
dc.date.issued
2019-01-12
dc.identifier.uri
http://hdl.handle.net/20.500.11850/355608
dc.identifier.doi
10.3929/ethz-b-000355608
dc.description.abstract
Grasses of the genera Lolium and Festuca are the main feed sources for a sustainable livestock production due to their high palatability and biomass production. Since decades, their importance for the agriculture of temperate regions led to the development of new varieties through traditional breeding programs. However, newer crop improvement methods such as genomic selection could benefit from a high quality reference genome assembly. In the past, attempts in delivering such a dataset have struggled due to the complexity of the genome and the high heterozygosity of individual genotypes. We sequenced an individual of the L. multiflorum (Italian ryegrass) cv. Rabiosa, producing a highly contiguous (N50 of 3 Mb) and complete assembly (97% of the BUSCO gene models). Due to the high heterozygosity of the line, the assembly (4.5 Gb) resulted to be as large as the diploid genome, and presented the sequence of both alleles in separate scaffolds. About ~70,000 gene models were identified, and the repeat content approached 80%. The comparison of a representative allelic region showed an extensive amount of intergenic sequence variation, supporting the high dynamicity of grass genomes. Compared to the available Lolium genome assemblies, the Rabiosa assembly improves contiguity by >40-fold, contains both haplotypes of the diploid parent, and assigns most of the sequence to chromosomes. The availability of a complete and highly-contiguous genome assembly of Italian ryegrass paves the road to the exploitation of the forage crops genetic resources by means of modern genomic platforms.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.rights.uri
http://rightsstatements.org/page/InC-NC/1.0/
dc.title
Characterization of a Lolium multiflorum diploid assembly
en_US
dc.type
Other Conference Item
dc.rights.license
In Copyright - Non-Commercial Use Permitted
ethz.pages.start
W591
en_US
ethz.size
4 p. published version; 19 p. accepted version
en_US
ethz.version.deposit
acceptedVersion
en_US
ethz.event
Plant and Animal Genome XXVII Conference (PAG XXVII 2019)
en_US
ethz.event.location
San Diego, CA, USA
en_US
ethz.event.date
January 12-16, 2019
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.identifier.url
https://plan.core-apps.com/pag_2019/abstract/05b01602-1d8a-40e8-a7b2-55a0d39f62f3
ethz.date.deposited
2019-07-29T11:43:00Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-07-29T13:19:43Z
ethz.rosetta.lastUpdated
2019-07-29T13:19:43Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Characterization%20of%20a%20Lolium%20multiflorum%20diploid%20assembly&rft.date=2019-01-12&rft.spage=W591&rft.au=Copetti,%20Dario&Kuon,%20Joel%E2%80%91Elias&Yates,%20Steven&K%C3%B6lliker,%20Roland&Studer,%20Bruno&rft.genre=unknown&rft.btitle=Characterization%20of%20a%20Lolium%20multiflorum%20diploid%20assembly
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