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dc.contributor.author
Frezel, Noémie
dc.contributor.author
Kratzer, Gilles
dc.contributor.author
Verzar, Philipp
dc.contributor.author
Bürki, Jérome
dc.contributor.author
Weber, Fabienne A.
dc.contributor.author
Zeilhofer, Hanns U.
dc.date.accessioned
2019-09-02T13:57:31Z
dc.date.available
2019-08-30T16:38:48Z
dc.date.available
2019-09-02T13:57:31Z
dc.date.issued
2019
dc.identifier.other
10.1097/PR9.0000000000000740
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/361610
dc.identifier.doi
10.3929/ethz-b-000361610
dc.description.abstract
Introduction: Genetically modified mice are widely used in studies on human and animal physiology and pharmacology, including pain research. The experimental design usually includes comparisons of genetically modified mice with wild-type littermates, requiring biopsy material for genotyping and methods for unequivocal identification of individual mice. Ethical standards and, in some countries, legislation require that both needs are reached with a single procedure. Clipping of the most distal phalanx of up to two toes per paw (toe clipping) is the favored procedure in most research fields, but it may be problematic in sensory physiology and pain research. Objectives: To systematically investigate whether toe-clipping influences later-in-life nociceptive sensitivity or the susceptibility to neuropathic or inflammatory hyperalgesia. Methods: We tested in male mice whether the clipping of 2 toes of a hind paw influences nociceptive sensitivities to noxious heat or cold, or to mechanical stimulation under baseline conditions, after peripheral nerve injury (chronic constriction of the sciatic nerve) or during peripheral inflammation induced by subcutaneous zymosan A injection. We tested not only for the presence of significant differences but also specifically addressed bioequivalence using the 2 one-sided t test procedure. We chose a threshold of 25% variation of the control value for nonequivalence, which is usually taken as a threshold for biological relevance in pain tests. Results: Using this value, we found that for all conditions (non-neuropathic and non-inflamed, neuropathic and inflamed), nociceptive sensitivities significantly fell within the equivalence bounds of the non–toe-clipped control mice. Conclusions: These results suggest that toe clipping does not have long-term effects on nociceptive sensitivities and does not alter the susceptibility of male mice to neuropathic or inflammatory hyperalgesia.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wolters Kluwer Health
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Mouse
en_US
dc.subject
Pain
en_US
dc.subject
Genotyping
en_US
dc.subject
Priming
en_US
dc.subject
Trauma
en_US
dc.subject
Nociception
en_US
dc.subject
Painmodel
en_US
dc.subject
Biopsy
en_US
dc.subject
Toe clipping
en_US
dc.subject
Surgery
en_US
dc.subject
Postoperative pain
en_US
dc.title
Does toe clipping for genotyping interfere with later-in-life nociception in mice?
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Pain Reports
ethz.journal.volume
4
en_US
ethz.journal.issue
3
en_US
ethz.pages.start
e740
en_US
ethz.size
7 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
Philadelphia, PA
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-08-30T16:38:55Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-09-02T13:57:42Z
ethz.rosetta.lastUpdated
2019-09-02T13:57:42Z
ethz.rosetta.versionExported
true
ethz.COinS
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