Microfluidic generation of amino-functionalized hydrogel microbeads capable of on-bead bioassay
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
Microfluidic generation of hydrogel microbeads is a highly efficient and reproducible approach to create various functional hydrogel beads. Here, we report a method to prepare crosslinked amino-functionalized polyethylene glycol (PEG) microbeads using a microfluidic channel. The microbeads generated from a microfluidic device were evaluated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and confocal laser scanning microscopy, respectively. We found that the microbeads were monodisperse and the amino groups were localized on the shell region of the microbeads. A swelling test exhibited compatibility with various solvents. A cell binding assay was successfully performed with RGD peptide-coupled amino-functionalized hydrogel microbeads. This strategy will enable the large production of the various functional microbeads, which can be used for solid phase peptide synthesis and on-bead bioassays. Show more
Journal / seriesMicromachines
Pages / Article No.
Subjectpolyethylene glycol; hydrogel microbead; microfluidic channel; on-bead bioassay; peptide synthesis
MoreShow all metadata