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dc.contributor.author
Chagneau, Camille V.
dc.contributor.author
Garcie, Christophe
dc.contributor.author
Bossuet-Greif, Nadège
dc.contributor.author
Tronnet, Sophie
dc.contributor.author
Brachmann, Alexander O.
dc.contributor.author
Piel, Jörn
dc.contributor.author
Nougayrède, Jean-Philippe
dc.contributor.author
Martin, Patricia
dc.contributor.author
Oswald, Eric
dc.date.accessioned
2019-10-15T09:05:12Z
dc.date.available
2019-10-15T02:41:39Z
dc.date.available
2019-10-15T09:05:12Z
dc.date.issued
2019
dc.identifier.other
10.1128/mSphere.00414-19
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/370492
dc.identifier.doi
10.3929/ethz-b-000370492
dc.description.abstract
Colibactin is a polyketide/nonribosomal peptide produced by Escherichia coli strains that harbor the pks island. This toxin induces DNA double-strand breaks and DNA interstrand cross-links in infected eukaryotic cells. Colibactin-producing strains are found associated with colorectal cancer biopsy specimens and promote intestinal tumor progression in various murine models. Polyamines are small polycationic molecules produced by both microorganisms and eukaryotic cells. Their levels are increased in malignancies, where they contribute to disease progression and metastasis. In this study, we demonstrated that the endogenous spermidine synthase SpeE is required for full genotoxic activity of colibactin-producing E. coli. Supplying spermidine in a ΔspeE pks+ E. coli strain restored genotoxic activity. Spermidine is involved in the autotoxicity linked to colibactin and is required for direct damaging activity on DNA. The production of the colibactin prodrug motif is impaired in ΔspeE mutants. Therefore, we demonstrated that spermidine has a direct impact on colibactin synthesis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Microbiology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Escherichia coli
en_US
dc.subject
biosynthesis
en_US
dc.subject
colorectal cancer
en_US
dc.subject
genotoxic colibactin
en_US
dc.subject
polyamines
en_US
dc.title
The Polyamine Spermidine Modulates the Production of the Bacterial Genotoxin Colibactin
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-10-02
ethz.journal.title
mSphere
ethz.journal.volume
4
en_US
ethz.journal.issue
5
en_US
ethz.pages.start
e00414-19
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-10-15T02:41:57Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-10-15T09:05:26Z
ethz.rosetta.lastUpdated
2019-10-15T09:05:26Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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