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dc.contributor.author
Generali, Melanie
dc.contributor.author
Casanova, Elisa A.
dc.contributor.author
Kehl, Debora
dc.contributor.author
Wanner, Debora
dc.contributor.author
Hoerstrup, Simon P.
dc.contributor.author
Cinelli, Paolo
dc.contributor.author
Weber, Benedikt
dc.date.accessioned
2019-10-21T10:44:14Z
dc.date.available
2019-10-20T03:03:14Z
dc.date.available
2019-10-21T10:44:14Z
dc.date.issued
2019-10-01
dc.identifier.other
10.1016/j.actbio.2019.07.032
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/371755
dc.identifier.doi
10.3929/ethz-b-000371755
dc.description.abstract
An ideal cell source for human therapeutic and disease modeling applications should be easily accessible and possess unlimited differentiation and expansion potential. Human induced pluripotent stem cells (hiPSCs) derived from peripheral blood mononuclear cells (PBMCs) represent a promising source given their ease of harvest and their pluripotent nature. Previous studies have demonstrated the feasibility of using PBMC-derived hiPSCs for vascular tissue engineering. However, so far, no endothelialization of hiPSC-derived tissue engineered vascular grafts (TEVGs) based on fully biodegradable polymers without xenogenic matrix components has been shown. In this study, we have generated hiPSCs from PBMCs and differentiated them into αSMA- and calponin-positive smooth muscle cells (SMCs) as well as endothelial cells (ECs) positive for CD31, vWF and eNOS. Both cell types were co-seeded on PGA-P4HB starter matrices and cultured under static or dynamic conditions to induce tissue formation in vitro. The resulting small diameter vascular grafts showed abundant amounts of extracellular matrix, containing a thin luminal layer of vWF-positive cells and a subendothelial αSMA-positive layer approximating the architecture of native vessels. Our results demonstrate the successful generation of TEVGs based on SMCs and ECs differentiated from PBMC-derived hiPSC combined with a biodegradable polymer. These results pave the way for developing autologous PBMC-derived hiPSC-based vascular constructs for therapeutic applications or disease modeling.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Peripheral blood mononuclear cells
en_US
dc.subject
Induced pluripotent stem cells
en_US
dc.subject
Vascular tissue engineering
en_US
dc.subject
Blood vessels
en_US
dc.subject
Biodegradable polymer
en_US
dc.title
Autologous endothelialized small-caliber vascular grafts engineered from blood-derived induced pluripotent stem cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2019-07-22
ethz.journal.title
Acta Biomaterialia
ethz.journal.volume
97
en_US
ethz.pages.start
333
en_US
ethz.pages.end
343
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-10-20T03:03:22Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-10-21T10:44:36Z
ethz.rosetta.lastUpdated
2020-02-15T22:08:38Z
ethz.rosetta.versionExported
true
ethz.COinS
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