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dc.contributor.author
Burkard, Michael
dc.contributor.author
Bengtson Nash, Susan
dc.contributor.author
Gambaro, Gessica
dc.contributor.author
Whitworth, Deanne
dc.contributor.author
Schirmer, Kristin
dc.date.accessioned
2019-10-29T08:43:42Z
dc.date.available
2019-10-28T15:58:07Z
dc.date.available
2019-10-29T08:43:42Z
dc.date.issued
2019-08
dc.identifier.issn
1573-6822
dc.identifier.issn
0742-2091
dc.identifier.other
10.1007/s10565-018-09457-1
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/373395
dc.identifier.doi
10.3929/ethz-b-000373395
dc.description.abstract
Marine mammals, such as whales, have a high proportion of body fat and so are susceptible to the accumulation, and associated detrimental health effects, of lipophilic environmental contaminants. Recently, we developed a wild-type cell line from humpback whale fibroblasts (HuWa). Extensive molecular assessments with mammalian wild-type cells are typically constrained by a finite life span, with cells eventually becoming senescent. Thus, the present work explored the possibility of preventing senescence in the HuWa cell line by transfection with plasmids encoding the simian virus large T antigen (SV40T) or telomerase reverse transcriptase (TERT). No stable expression was achieved upon SV40 transfection. Transfection with TERT, on the other hand, activated the expression of telomerase in HuWa cells. At the time of manuscript preparation, the transfected HuWa cells (HuWaTERT) have been stable for at least 59 passages post-transfection. HuWaTERT proliferate rapidly and maintain initial cell characteristics, such as morphology and chromosomal stability. The response of HuWaTERT cells to an immune stimulant (lipopolysaccharide (LPS)) and an immunotoxicant (Aroclor1254) was assessed by measurement of intracellular levels of the pro-inflammatory cytokines interleukin (IL)-6, IL-1β and tumour necrosis factor (TNF)-α. HuWaTERT cells constitutively express IL-6, IL-1β and TNFα. Exposure to neither LPS nor Aroclor1254 had an effect on the levels of these cytokines. Overall, this work supports the diverse applicability of HuWa cell lines in that they display reliable long-term preservation, susceptibility to exogenous gene transfer and enable the study of humpback whale-specific cellular response mechanisms.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Cell line transfection
en_US
dc.subject
Humpback whale
en_US
dc.subject
Megaptera novaeangliae
en_US
dc.subject
Immunotoxicity
en_US
dc.subject
Inflammatory cytokines
en_US
dc.subject
Relative telomerase activity
en_US
dc.title
Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor)
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-01-10
ethz.journal.title
Cell Biology and Toxicology
ethz.journal.volume
35
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
Cell Biol Toxicol
ethz.pages.start
387
en_US
ethz.pages.end
398
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Berlin
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-10-28T15:58:17Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-10-29T08:44:06Z
ethz.rosetta.lastUpdated
2022-03-28T23:58:47Z
ethz.rosetta.versionExported
true
ethz.COinS
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