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dc.contributor.author
Bergmiller, Tobias
dc.contributor.author
Peña-Miller, Rafael
dc.contributor.author
Boehm, Alexander
dc.contributor.author
Ackermann, Martin
dc.date.accessioned
2019-04-26T13:56:04Z
dc.date.available
2017-06-09T11:57:02Z
dc.date.available
2019-04-26T13:53:52Z
dc.date.available
2019-04-26T13:56:04Z
dc.date.issued
2011
dc.identifier.issn
1471-2180
dc.identifier.other
10.1186/1471-2180-11-118
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/37457
dc.identifier.doi
10.3929/ethz-b-000037457
dc.description.abstract
Background The essential Escherichia coli gene ygjD belongs to a universally conserved group of genes whose function has been the focus of a number of recent studies. Here, we put ygjD under control of an inducible promoter, and used time-lapse microscopy and single cell analysis to investigate the phenotypic consequences of the depletion of YgjD protein from growing cells. Results We show that loss of YgjD leads to a marked decrease in cell size and termination of cell division. The transition towards smaller size occurs in a controlled manner: cell elongation and cell division remain coupled, but cell size at division decreases. We also find evidence that depletion of YgjD leads to the synthesis of the intracellular signaling molecule (p)ppGpp, inducing a cellular reaction resembling the stringent response. Concomitant deletion of the relA and spoT genes - leading to a strain that is uncapable of synthesizing (p)ppGpp - abrogates the decrease in cell size, but does not prevent termination of cell division upon YgjD depletion. Conclusions Depletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division. The combination of single-cell timelapse microscopy and statistical analysis can give detailed insights into the phenotypic consequences of the loss of essential genes, and can thus serve as a new tool to study the function of essential genes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
araC
en_US
dc.subject
Essential Gene
en_US
dc.subject
Elongation Rate
en_US
dc.subject
Stringent Response
en_US
dc.subject
Sister Cell
en_US
dc.title
Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
dc.date.published
2011-05-27
ethz.journal.title
BMC Microbiology
ethz.journal.volume
11
en_US
ethz.journal.abbreviated
BMC microbiol. (Online)
ethz.pages.start
118
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004100852
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::03743 - Ackermann, Martin / Ackermann, Martin
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::03743 - Ackermann, Martin / Ackermann, Martin
ethz.date.deposited
2017-06-09T11:57:18Z
ethz.source
ECIT
ethz.identifier.importid
imp59364e3a70bb038046
ethz.ecitpid
pub:59975
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T18:26:52Z
ethz.rosetta.lastUpdated
2019-04-26T13:56:08Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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