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dc.contributor.author
Pinotsi, Dorothea
dc.contributor.author
Rodighiero, Simona
dc.contributor.author
Campioni, Silvia
dc.contributor.author
Csucs, Gabor
dc.date.accessioned
2019-11-08T10:40:06Z
dc.date.available
2019-11-08T03:46:18Z
dc.date.available
2019-11-08T10:40:06Z
dc.date.issued
2019
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/s41598-019-52047-2
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/376127
dc.identifier.doi
10.3929/ethz-b-000376127
dc.description.abstract
A number of new Correlative Light and Electron Microscopy approaches have been developed over the past years, offering the opportunity to combine the specificity and bio-compatibility of light microscopy with the high resolution achieved in electron microscopy. More recently, these approaches have taken one step further and also super-resolution light microscopy was combined with transmission or scanning electron microscopy. This combination usually requires moving the specimen between different imaging systems, an expensive set-up and relatively complicated imaging workflows. Here we present a way to overcome these difficulties by exploiting a commercially available wide-field fluorescence microscope integrated in the specimen chamber of a Scanning Electron Microscope (SEM) to perform correlative LM/EM studies. Super-resolution light microscopy was achieved by using a recently developed algorithm - the Super-Resolution Radial Fluctuations (SRRF) - to improve the resolution of diffraction limited fluorescent images. With this combination of hardware/software it is possible to obtain correlative super-resolution light and scanning electron microscopy images in an easy and fast way. The imaging workflow is described and demonstrated on fluorescently labelled amyloid fibrils, fibrillar protein aggregates linked to the onset of multiple neurodegenerative diseases, revealing information about their polymorphism.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
An Easy Path for Correlative Electron and Super-Resolution Light Microscopy
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-10-29
ethz.journal.title
Scientific Reports
ethz.journal.volume
9
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
15526
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02891 - ScopeM / ScopeM
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02891 - ScopeM / ScopeM
ethz.date.deposited
2019-11-08T03:46:25Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-02-15T22:25:43Z
ethz.rosetta.lastUpdated
2021-02-15T06:38:12Z
ethz.rosetta.versionExported
true
ethz.COinS
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