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dc.contributor.author
Jay, Florence
dc.contributor.author
Wang, Yu
dc.contributor.author
Yu, Agnès
dc.contributor.author
Taconnat, Ludivine
dc.contributor.author
Pelletier, Sandra
dc.contributor.author
Colot, Vincent
dc.contributor.author
Renou, Jean-Pierre
dc.contributor.author
Voinnet, Olivier
dc.date.accessioned
2019-08-27T09:06:20Z
dc.date.available
2017-06-09T12:03:38Z
dc.date.available
2019-08-27T08:24:28Z
dc.date.available
2019-08-27T09:03:18Z
dc.date.available
2019-08-27T09:06:20Z
dc.date.issued
2011-05-12
dc.identifier.other
10.1371/journal.ppat.1002035
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/37735
dc.identifier.doi
10.3929/ethz-b-000037735
dc.description.abstract
In Arabidopsis, micro (mi)RNAs and trans-acting (ta-si)RNAs synthesized directly or indirectly through the DICER-LIKE-1 (DCL1) ribonuclease have roles in patterning and hormonal responses, while DCL2,3,4-dependent small-interfering (si)RNAs are mainly involved in silencing of transposable elements and antiviral defense. Viral suppressors of RNA silencing (VSRs) produced by phytoviruses to counter plant defense may perturb plant developmental programs because of the collision of their inhibitory effects with the regulatory action of endogenous miRNAs and ta-siRNAs. This could explain the similar developmental aberrations displayed by Arabidopsis miRNA/ta-siRNA pathway mutants, including dcl1, and by some VSR-expressing plants. Nonetheless, the molecular bases for these morphological aberrations have remained mysterious, and their contribution to viral disease symptoms/virulence unexplored. The extent of VSR inhibitory actions to other types of endogenous small RNAs remains also unclear. Here, we present an in-depth analysis of transgenic Arabidopsis expressing constitutively HcPro, P19 and P15, three unrelated VSRs. We show that VSR expression has comparable, yet modest effects on known miRNA and ta-siRNA target RNA levels, similar to those observed using an hypomorphic dcl1 mutation. However, by combining results of transcriptome studies with deep-sequencing data from immuno-precipitated small RNAs, additional, novel endogenous targets of miRNA and ta-siRNA were identified, unraveling an unsuspected complexity in the origin and scope-of-action of these molecules. Other stringent analyses pinpointed misregulation of the miR167 target AUXIN RESPONSE FACTOR 8 (ARF8) as a major cause for the developmental aberrations exhibited by VSR transgenic plants, but also for the phenotypes induced during normal viral infection caused by the HcPro-encoding Turnip mosaic virus (TuMV). Neither RNA silencing, its suppression by VSRs, nor the virulence/accumulation of TuMV was altered by mutations in ARF8. These findings have important implications for our understanding of viral disease symptoms and small RNA-directed regulation of plant growth/development.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Misregulation of AUXIN RESPONSE FACTOR 8 Underlies the Developmental Abnormalities Caused by Three Distinct Viral Silencing Suppressors in Arabidopsis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS pathogens
ethz.journal.volume
7
en_US
ethz.journal.issue
5
en_US
ethz.pages.start
e1002035
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.notes
A Correction has been published.
en_US
ethz.identifier.wos
ethz.identifier.nebis
005409548
ethz.publication.place
Lawrence, KS
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03876 - Voinnet, Olivier / Voinnet, Olivier
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03876 - Voinnet, Olivier / Voinnet, Olivier
ethz.relation.isReferencedBy
10.3929/ethz-b-000360681
ethz.date.deposited
2017-06-09T12:04:01Z
ethz.source
ECIT
ethz.identifier.importid
imp59364e3fedf0942607
ethz.ecitpid
pub:60276
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T09:38:58Z
ethz.rosetta.lastUpdated
2019-08-27T09:06:38Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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