Show simple item record

dc.contributor.author
Zhang, Huoming
dc.contributor.author
Liu, Pei
dc.contributor.author
Guo, Tiannan
dc.contributor.author
Zhao, Huayan
dc.contributor.author
Bensaddek, Dalila
dc.contributor.author
Aebersold, Ruedi
dc.contributor.author
Xiong, Liming
dc.date.accessioned
2019-12-02T09:40:47Z
dc.date.available
2019-12-02T03:40:20Z
dc.date.available
2019-12-02T09:40:47Z
dc.date.issued
2019-11-22
dc.identifier.other
10.1038/s41597-019-0294-0
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/381779
dc.identifier.doi
10.3929/ethz-b-000381779
dc.description.abstract
Arabidopsis is an important model organism and the first plant with its genome completely sequenced. Knowledge from studying this species has either direct or indirect applications for agriculture and human health. Quantitative proteomics by data-independent acquisition mass spectrometry (SWATH/DIA-MS) was recently developed and is considered as a high-throughput, massively parallel targeted approach for accurate proteome quantification. In this approach, a high-quality and comprehensive spectral library is a prerequisite. Here, we generated an expression atlas of 10 organs of Arabidopsis and created a library consisting of 15,514 protein groups, 187,265 unique peptide sequences, and 278,278 precursors. The identified protein groups correspond to ~56.5% of the predicted proteome. Further proteogenomics analysis identified 28 novel proteins. We applied DIA-MS using this library to quantify the effect of abscisic acid on Arabidopsis. We were able to recover 8,793 protein groups of which 1,787 were differentially expressed. MS data are available via ProteomeXchange with identifier PXD012708 and PXD012710 for data-dependent acquisition and PXD014032 for DIA analyses.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Arabidopsis proteome and the mass spectral assay library
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific data
ethz.journal.volume
6
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
278
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-12-02T03:40:26Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-12-02T09:41:02Z
ethz.rosetta.lastUpdated
2019-12-02T09:41:02Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Arabidopsis%20proteome%20and%20the%20mass%20spectral%20assay%20library&rft.jtitle=Scientific%20data&rft.date=2019-11-22&rft.volume=6&rft.issue=1&rft.spage=278&rft.au=Zhang,%20Huoming&Liu,%20Pei&Guo,%20Tiannan&Zhao,%20Huayan&Bensaddek,%20Dalila&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record