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dc.contributor.author
Wiatr, Marie
dc.contributor.author
Stump-Guthier, Carolin
dc.contributor.author
Latorre, Daniela
dc.contributor.author
Uhlig, Stefanie
dc.contributor.author
Weiss, Christel
dc.contributor.author
Ilonen, Jorma
dc.contributor.author
Engelhardt, Britta
dc.contributor.author
Ishikawa, Hiroshi
dc.contributor.author
Schwerk, Christian
dc.contributor.author
Schroten, Horst
dc.contributor.author
Tenenbaum, Tobias
dc.contributor.author
Rudolph, Henriette
dc.date.accessioned
2019-12-02T09:36:20Z
dc.date.available
2019-12-02T03:40:20Z
dc.date.available
2019-12-02T09:36:20Z
dc.date.issued
2019-11-21
dc.identifier.issn
1742-2094
dc.identifier.other
10.1186/s12974-019-1626-x
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/381781
dc.identifier.doi
10.3929/ethz-b-000381781
dc.description.abstract
Background Echovirus 30 (E-30) is one of the most frequently isolated pathogens in aseptic meningitis worldwide. To gain access to the central nervous system (CNS), E-30 and immune cells have to cross one of the two main barriers of the CNS, the epithelial blood–cerebrospinal fluid barrier (BCSFB) or the endothelial blood–brain barrier (BBB). In an in vitro model of the BCSFB, it has been shown that E-30 can infect human immortalized brain choroid plexus papilloma (HIBCPP) cells. Methods In this study we investigated the migration of different T cell subpopulations, naive and effector T cells, through HIBCPP cells during E-30 infection. Effects of E-30 infection and the migration process were evaluated via immunofluorescence and flow cytometry analysis, as well as transepithelial resistance and dextran flux measurement. Results Th1 effector cells and enterovirus-specific effector T cells migrated through HIBCPP cells more efficiently than naive CD4+ T cells following E-30 infection of HIBCPP cells. Among the different naive T cell populations, CD8+ T cells crossed the E-30-infected HIBCPP cell layer in a significantly higher number than CD4+ T cells. A large amount of effector T cells also remained attached to the basolateral side of the HIBCPP cells compared with naive T cells. Analysis of HIBCPP barrier function showed significant alteration after E-30 infection and trans- as well as paracellular migration of T cells independent of the respective subpopulation. Morphologic analysis of migrating T cells revealed that a polarized phenotype was induced by the chemokine CXCL12, but reversed to a round phenotype after E-30 infection. Further characterization of migrating Th1 effector cells revealed a downregulation of surface adhesion proteins such as LFA-1 PSGL-1, CD44, and CD49d. Conclusion Taken together these results suggest that naive CD8+ and Th1 effector cells are highly efficient to migrate through the BCSFB in an inflammatory environment. The T cell phenotype is modified during the migration process through HIBCPP cells.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Enterovirus
en_US
dc.subject
Blood–cerebrospinal fluid barrier
en_US
dc.subject
T cell migration
en_US
dc.subject
Effector T cells
en_US
dc.subject
Naive T cells
en_US
dc.subject
Meningitis
en_US
dc.title
Distinct migratory pattern of naive and effector T cells through the blood–CSF barrier following Echovirus 30 infection
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Journal of neuroinflammation
ethz.journal.volume
16
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
J. neuroinflamm.
ethz.pages.start
232
en_US
ethz.size
19 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2019-12-02T03:40:26Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-12-02T09:36:33Z
ethz.rosetta.lastUpdated
2019-12-02T09:36:33Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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