Show simple item record

dc.contributor.author
Arigoni-Affolter, Ilaria
dc.contributor.author
Scibona, Ernesto
dc.contributor.author
Lin, Chia-Wei
dc.contributor.author
Brühlmann, David
dc.contributor.author
Souquet, Jonathan
dc.contributor.author
Broly, Hervé
dc.contributor.author
Aebi, Markus
dc.date.accessioned
2019-12-11T13:44:13Z
dc.date.available
2019-12-11T11:07:45Z
dc.date.available
2019-12-11T13:44:13Z
dc.date.issued
2019-11-01
dc.identifier.issn
2375-2548
dc.identifier.other
10.1126/sciadv.aax8930
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/384632
dc.identifier.doi
10.3929/ethz-b-000384632
dc.description.abstract
N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes. The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway. We present a glycoproteomics approach to monitor the processing of site-specific N-glycans in CHO cells. On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway. This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
AAAS
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.title
Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
dc.date.published
2019-11-27
ethz.journal.title
Science Advances
ethz.journal.volume
5
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
Sci Adv
ethz.pages.start
eaax8930
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
N-linked protein glycosylation: generating diversity
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03408 - Aebi, Markus (emeritus) / Aebi, Markus (emeritus)
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03408 - Aebi, Markus (emeritus) / Aebi, Markus (emeritus)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.grant.agreementno
162636
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2019-12-11T11:07:59Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2019-12-11T13:44:29Z
ethz.rosetta.lastUpdated
2021-02-15T07:03:18Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Mechanistic%20reconstruction%20of%20glycoprotein%20secretion%20through%20monitoring%20of%20intracellular%20N-glycan%20processing&rft.jtitle=Science%20Advances&rft.date=2019-11-01&rft.volume=5&rft.issue=11&rft.spage=eaax8930&rft.issn=2375-2548&rft.au=Arigoni-Affolter,%20Ilaria&Scibona,%20Ernesto&Lin,%20Chia-Wei&Br%C3%BChlmann,%20David&Souquet,%20Jonathan&rft.genre=article&rft_id=info:doi/10.1126/sciadv.aax8930&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record