Open access
Datum
2019-12-05Typ
- Journal Article
ETH Bibliographie
yes
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Abstract
Condensin is a conserved SMC complex that uses its ATPase machinery to structure genomes, but how it does so is largely unknown. We show that condensin’s ATPase has a dual role in chromosome condensation. Mutation of one ATPase site impairs condensation, while mutating the second site results in hyperactive condensin that compacts DNA faster than wild-type, both in vivo and in vitro. Whereas one site drives loop formation, the second site is involved in the formation of more stable higher-order Z loop structures. Using hyperactive condensin I, we reveal that condensin II is not intrinsically needed for the shortening of mitotic chromosomes. Condensin II rather is required for a straight chromosomal axis and enables faithful chromosome segregation by counteracting the formation of ultrafine DNA bridges. SMC complexes with distinct roles for each ATPase site likely reflect a universal principle that enables these molecular machines to intricately control chromosome architecture. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000386421Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Molecular CellBand
Seiten / Artikelnummer
Verlag
Cell PressETH Bibliographie
yes
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