A multiplexed cell-free assay in double emulsion droplets

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Author
Nuti, Nicola
Rottmann, Philipp
Stucki, Ariane
Krähenbühl, Sven
Dittrich, Petra S.cc
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Date
2019
Type
    Other Conference Item
Abstract
We introduce a novel on-chip assay, performed in water-in-oil-in-water double emulsions, that uses cell-free protein synthesis and artificial liposomes for the screening of membrane disrupting toxin DNA libraries according to their antimicrobial activity and host membrane safety. Our method represe Show more
Publication status
published
Book title
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)
Pages / Article No.
510 - 511
Publisher
Chemical and Biological Microsystems Society
Event
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019), Basel, Switzerland, October 27-31, 2019
Subject
liposomes; cell-free; double-emulsions; antimicrobial peptides; membrane disrupting toxins
Organisational unit
03807 - Dittrich, Petra / Dittrich, Petra  
Funding
681587 - Engineering of hybrid cells using lab-on-chip technology (EC)
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yes
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