ITO Substrates for CLEM of Fibril Proteins: Tips & Tricks for Scanning Electron and Fluorescence Microscopy

Abstract

Correlative light and electron microscopy in a SEM is usually performed using glass commercial substrates coated with indium-thin oxide. These substrates however show a patchy background unavoidably imaged when fibril proteins are deposited on them. To avoid this effect, keeping both optical transparency and electrical conductivity of the same kind of substrates, we propose two cheap and easy preparation methods by either magnetron sputtering or ion beam coating.