Identifying extracellular vesicle populations from long-term cultured single cells using multi-color TIRFM

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Author
Nikoloff, Jonas
Armbrecht, Lucas
Kling, André
Dittrich, Petra S.cc
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Date
2019
Type
    Other Conference Item
Abstract
We here report a double-layer microfluidic device for detecting and identifying secreted extracellular vesicle populations, among them exosomes, from single long-term cultured cells using multi-color Total Internal Reflection Fluorescence Microscopy (TIRFM). Using a custom-developed MATLAB script f Show more
Publication status
published
Book title
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)
Pages / Article No.
70 - 71
Publisher
Chemical and Biological Microsystems Society
Event
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019), Basel, Switzerland, October 27-31, 2019
Subject
Extracellular vesicles; single-cell analysis; TIRFM
Organisational unit
03807 - Dittrich, Petra / Dittrich, Petra  
Funding
681587 - Engineering of hybrid cells using lab-on-chip technology (EC)
Notes
Conference lecture on October 29, 2019.
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