Identifying extracellular vesicle populations from long-term cultured single cells using multi-color TIRFM
- Other Conference Item
We here report a double-layer microfluidic device for detecting and identifying secreted extracellular vesicle populations, among them exosomes, from single long-term cultured cells using multi-color Total Internal Reflection Fluorescence Microscopy (TIRFM). Using a custom-developed MATLAB script for image processing we achieved quantitative population analysis of vesicles secreted from single Michigan Cancer Foundation (MCF)-7 cells cultivated for 2-5 days Show more
Book title23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectExtracellular vesicles; single-cell analysis; TIRFM
Organisational unit03807 - Dittrich, Petra / Dittrich, Petra
681587 - Engineering of hybrid cells using lab-on-chip technology (EC)
NotesConference lecture on October 29, 2019.
MoreShow all metadata