Show simple item record

dc.contributor.author
Del Medico, Luca
dc.contributor.author
Cerletti, Dario
dc.contributor.author
Christen, Matthias
dc.contributor.author
Christen, Beat
dc.date.accessioned
2020-02-10T14:45:32Z
dc.date.available
2020-01-24T14:53:57Z
dc.date.available
2020-02-10T14:45:32Z
dc.date.issued
2019-09-27
dc.identifier.other
10.1101/766329
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/393899
dc.identifier.doi
10.3929/ethz-b-000393899
dc.description.abstract
Understanding how bacteria colonize surfaces and regulate cell cycle progression in response to cellular adhesion is of fundamental importance. Here, we used transposon sequencing in conjunction with FRET microscopy to uncover the molecular mechanism how surface sensing drives cell cycle initiation in Caulobacter crescentus. We identified the type IV pilin protein PilA as the primary signaling input that couples surface contact to cell cycle initiation via the second messenger c-di-GMP. Upon retraction of pili filaments, the monomeric pilin reservoir in the inner membrane is sensed by the 17 amino-acid transmembrane helix of PilA to activate the PleC-PleD two component signaling system, increase cellular c-di-GMP levels and signal the onset of the cell cycle. We termed the PilA signaling sequence CIP for cell cycle initiating pilin peptide. Addition of the chemically synthesized CIP peptide initiates cell cycle progression and simultaneously inhibits surface attachment. The broad conservation of the type IV pili and their importance in pathogens for host colonization suggests that CIP peptide mimetics offer new strategies to inhibit surface-sensing, prevent biofilm formation and control persistent infections.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Cold Spring Harbor Laboratory
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
cell cycle
en_US
dc.subject
Type IV pili biogenesis
en_US
dc.subject
TnSeq
en_US
dc.subject
surface sensing
en_US
dc.title
The type IV pilin PilA couples surface attachment and cell cycle initiation in Caulobacter crescentus
en_US
dc.type
Working Paper
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
ethz.journal.title
bioRxiv
ethz.size
17 p.
en_US
ethz.code.ddc
DDC::5 - Science
en_US
ethz.grant
Global identification and characterization of essential genome features by random transposon mutagenesis
en_US
ethz.grant
Chemical synthesis rewriting of a bacterial genome
en_US
ethz.grant
Intermicrobial and host-microbial interactions that determine the trajectory of mammalian microbial colorization in early life
en_US
ethz.publication.place
Cold Spring Harbor, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::09461 - Christen, Beat (ehemalig) / Christen, Beat (former)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::09461 - Christen, Beat (ehemalig) / Christen, Beat (former)
en_US
ethz.grant.agreementno
166476
ethz.grant.agreementno
184664
ethz.grant.agreementno
177164
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projekte Lebenswissenschaften
ethz.grant.program
Projekte Lebenswissenschaften
ethz.grant.program
Sinergia
ethz.date.deposited
2020-01-24T14:54:05Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-02-10T14:45:43Z
ethz.rosetta.lastUpdated
2022-03-29T00:57:25Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=The%20type%20IV%20pilin%20PilA%20couples%20surface%20attachment%20and%20cell%20cycle%20initiation%20in%20Caulobacter%20crescentus&rft.jtitle=bioRxiv&rft.date=2019-09-27&rft.au=Del%20Medico,%20Luca&Cerletti,%20Dario&Christen,%20Matthias&Christen,%20Beat&rft.genre=preprint&rft_id=info:doi/10.1101/766329&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record