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dc.contributor.author
Farrah, Terry
dc.contributor.author
Deutsch, Eric W.
dc.contributor.author
Omenn, Gilbert S.
dc.contributor.author
Campbell, David S.
dc.contributor.author
Sun, Zhi
dc.contributor.author
Bletz, Julie A.
dc.contributor.author
Mallick, Parag
dc.contributor.author
Katz, Jonathan E.
dc.contributor.author
Malmström, Johan
dc.contributor.author
Ossola, Reto
dc.contributor.author
Watts, Julian D.
dc.contributor.author
Lin, Biaoyang
dc.contributor.author
Zhang, Hui
dc.contributor.author
Moritz, Robert L.
dc.contributor.author
Aebersold, Ruedi
dc.date.accessioned
2020-07-13T06:50:59Z
dc.date.available
2017-06-09T13:38:08Z
dc.date.available
2020-07-13T06:50:59Z
dc.date.issued
2011-09
dc.identifier.issn
1535-9476
dc.identifier.issn
1535-9484
dc.identifier.other
10.1074/mcp.M110.006353
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/39494
dc.description.abstract
Human blood plasma can be obtained relatively noninvasively and contains proteins from most, if not all, tissues of the body. Therefore, an extensive, quantitative catalog of plasma proteins is an important starting point for the discovery of disease biomarkers. In 2005, we showed that different proteomics measurements using different sample preparation and analysis techniques identify significantly different sets of proteins, and that a comprehensive plasma proteome can be compiled only by combining data from many different experiments. Applying advanced computational methods developed for the analysis and integration of very large and diverse data sets generated by tandem MS measurements of tryptic peptides, we have now compiled a high-confidence human plasma proteome reference set with well over twice the identified proteins of previous high-confidence sets. It includes a hierarchy of protein identifications at different levels of redundancy following a clearly defined scheme, which we propose as a standard that can be applied to any proteomics data set to facilitate cross-proteome analyses. Further, to aid in development of blood-based diagnostics using techniques such as selected reaction monitoring, we provide a rough estimate of protein concentrations using spectral counting. We identified 20,433 distinct peptides, from which we inferred a highly nonredundant set of 1929 protein sequences at a false discovery rate of 1%. We have made this resource available via PeptideAtlas, a large, multiorganism, publicly accessible compendium of peptides identified in tandem MS experiments conductedby laboratories around the world. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Biochemistry and Molecular Biology
en_US
dc.title
A High-Confidence Human Plasma Proteome Reference Set with Estimated Concentrations in PeptideAtlas
en_US
dc.type
Journal Article
dc.date.published
2011-09-08
ethz.journal.title
Molecular & Cellular Proteomics
ethz.journal.volume
10
en_US
ethz.journal.issue
9
en_US
ethz.journal.abbreviated
Mol. cell. proteom.
ethz.pages.start
M110.006353
en_US
ethz.size
14 p.
en_US
ethz.identifier.wos
ethz.publication.place
Bethesda, MD
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus)
ethz.date.deposited
2017-06-09T13:38:41Z
ethz.source
ECIT
ethz.identifier.importid
imp59364e634f53a52775
ethz.ecitpid
pub:63635
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-18T14:35:18Z
ethz.rosetta.lastUpdated
2020-07-13T06:51:09Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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