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dc.contributor.author
Bhatnagar, Srijak
dc.contributor.author
Cowley, Elise S.
dc.contributor.author
Kopf, Sebastian H.
dc.contributor.author
Pérez Castro, Sherlynette
dc.contributor.author
Kearney, Sean M.
dc.contributor.author
Dawson, Scott C.
dc.contributor.author
Hanselmann, Kurt
dc.contributor.author
Ruff, S. Emil
dc.date.accessioned
2020-02-05T11:04:56Z
dc.date.available
2020-01-30T12:33:04Z
dc.date.available
2020-02-05T11:04:56Z
dc.date.issued
2020
dc.identifier.other
10.1186/s40793-019-0348-0
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/395834
dc.identifier.doi
10.3929/ethz-b-000395834
dc.description.abstract
Background Lagoons are common along coastlines worldwide and are important for biogeochemical element cycling, coastal biodiversity, coastal erosion protection and blue carbon sequestration. These ecosystems are frequently disturbed by weather, tides, and human activities. Here, we investigated a shallow lagoon in New England. The brackish ecosystem releases hydrogen sulfide particularly upon physical disturbance, causing blooms of anoxygenic sulfur-oxidizing phototrophs. To study the habitat, microbial community structure, assembly and function we carried out in situ experiments investigating the bloom dynamics over time. Results Phototrophic microbial mats and permanently or seasonally stratified water columns commonly contain multiple phototrophic lineages that coexist based on their light, oxygen and nutrient preferences. We describe similar coexistence patterns and ecological niches in estuarine planktonic blooms of phototrophs. The water column showed steep gradients of oxygen, pH, sulfate, sulfide, and salinity. The upper part of the bloom was dominated by aerobic phototrophic Cyanobacteria, the middle and lower parts by anoxygenic purple sulfur bacteria (Chromatiales) and green sulfur bacteria (Chlorobiales), respectively. We show stable coexistence of phototrophic lineages from five bacterial phyla and present metagenome-assembled genomes (MAGs) of two uncultured Chlorobaculum and Prosthecochloris species. In addition to genes involved in sulfur oxidation and photopigment biosynthesis the MAGs contained complete operons encoding for terminal oxidases. The metagenomes also contained numerous contigs affiliating with Microviridae viruses, potentially affecting Chlorobi. Our data suggest a short sulfur cycle within the bloom in which elemental sulfur produced by sulfide-oxidizing phototrophs is most likely reduced back to sulfide by Desulfuromonas sp. Conclusions The release of sulfide creates a habitat selecting for anoxygenic sulfur-oxidizing phototrophs, which in turn create a niche for sulfur reducers. Strong syntrophism between these guilds apparently drives a short sulfur cycle that may explain the rapid development of the bloom. The fast growth and high biomass yield of Chlorobi-affiliated organisms implies that the studied lineages of green sulfur bacteria can thrive in hypoxic habitats. This oxygen tolerance is corroborated by oxidases found in MAGs of uncultured Chlorobi. The findings improve our understanding of the ecology and ecophysiology of anoxygenic phototrophs and their impact on the coupled biogeochemical cycles of sulfur and carbon.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Microbial succession
en_US
dc.subject
Green sulfur bacteria
en_US
dc.subject
Prosthecochloris
en_US
dc.subject
Syntrophy
en_US
dc.subject
Brackish coastal ecosystem
en_US
dc.subject
Anoxygenic phototrophy
en_US
dc.subject
Microviridae
en_US
dc.subject
Sulfur cycling
en_US
dc.subject
CRISPR-Cas
en_US
dc.subject
Resilience
en_US
dc.title
Microbial community dynamics and coexistence in a sulfide-driven phototrophic bloom
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-01-17
ethz.journal.title
Environmental Microbiomes
ethz.journal.volume
15
en_US
ethz.pages.start
3
en_US
ethz.size
17 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.date.deposited
2020-01-30T12:33:24Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-02-05T11:05:07Z
ethz.rosetta.lastUpdated
2021-02-15T07:49:17Z
ethz.rosetta.versionExported
true
ethz.COinS
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