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dc.contributor.author
Eberl, Claudia
dc.contributor.author
Ring, Diana
dc.contributor.author
Münch, Philipp C.
dc.contributor.author
Beutler, Markus
dc.contributor.author
Basic, Marijana
dc.contributor.author
Slack, Emma
dc.contributor.author
Schwarzer, Martin
dc.contributor.author
Srutkova, Dagmar
dc.contributor.author
Lange, Anna
dc.contributor.author
Frick, Julia S.
dc.contributor.author
Bleich, André
dc.contributor.author
Stecher, Bärbel
dc.date.accessioned
2020-02-04T09:14:42Z
dc.date.available
2020-02-02T14:12:32Z
dc.date.available
2020-02-04T09:14:42Z
dc.date.issued
2020-01
dc.identifier.issn
1664-302X
dc.identifier.other
10.3389/fmicb.2019.02999
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/396512
dc.identifier.doi
10.3929/ethz-b-000396512
dc.description.abstract
The Oligo-Mouse-Microbiota (OMM12) is a recently developed synthetic bacterial community for functional microbiome research in mouse models (Brugiroux et al., 2016). To date, the OMM12 model has been established in several germ-free mouse facilities world-wide and is employed to address a growing variety of research questions related to infection biology, mucosal immunology, microbial ecology and host-microbiome metabolic cross-talk. The OMM12 consists of 12 sequenced and publically available strains isolated from mice, representing five bacterial phyla that are naturally abundant in the murine gastrointestinal tract (Lagkouvardos et al., 2016). Under germ-free conditions, the OMM12 colonizes mice stably over multiple generations. Here, we investigated whether stably colonized OMM12 mouse lines could be reproducibly established in different animal facilities. Germ-free C57Bl/6J mice were inoculated with a frozen mixture of the OMM12 strains. Within 2 weeks after application, the OMM12 community reached the same stable composition in all facilities, as determined by fecal microbiome analysis. We show that a second application of the OMM12 strains after 72 h leads to a more stable community composition than a single application. The availability of such protocols for reliable de novo generation of gnotobiotic rodents will certainly contribute to increasing experimental reproducibility in biomedical research.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
syncom
en_US
dc.subject
Oligo-MM12
en_US
dc.subject
sDMDMm2
en_US
dc.subject
minimal microbiome
en_US
dc.subject
3R
en_US
dc.subject
gnotobiology
en_US
dc.subject
defined bacterial consortia
en_US
dc.subject
isobiotic mice
en_US
dc.title
Reproducible Colonization of Germ-Free Mice With the Oligo-Mouse-Microbiota in Different Animal Facilities
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-01-10
ethz.journal.title
Frontiers in Microbiology
ethz.journal.volume
10
en_US
ethz.journal.abbreviated
Front Microbiol
ethz.pages.start
2999
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09640 - Wetter Slack, Emma / Wetter Slack, Emma
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09640 - Wetter Slack, Emma / Wetter Slack, Emma
ethz.date.deposited
2020-02-02T14:12:36Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-02-04T09:14:53Z
ethz.rosetta.lastUpdated
2024-02-02T10:18:40Z
ethz.rosetta.versionExported
true
ethz.COinS
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