Reuterin demonstrates potent antimicrobial activity against a broad panel of human and poultry meat Campylobacter spp. isolates

Open access
Author
Show all
Date
2020Type
- Journal Article
Citations
Cited 24 times in
Web of Science
Cited 24 times in
Scopus
ETH Bibliography
yes
Altmetrics
Abstract
Reuterin is a broad-spectrum antimicrobial system produced by specific strains of Lactobacillus reuteri during anaerobic metabolism of glycerol. Acrolein is the main component responsible for its antimicrobial activity. Here, the sensitivity of Campylobacter jejuni (n = 51) and Campylobacter coli (n = 20) isolates from chicken meat and human stool samples to reuterin was investigated. The minimum inhibitory concentration (MIC) of C. jejuni and C. coli strains was measured between 1.5 and 3.0 µM of acrolein, below the MIC of the sensitive indicator strain Escherichia coli K12 (16.5 µM acrolein). The interaction of C. jejuni N16-1419 and the reuterin-producing L. reuteri PTA5_F13 was studied during 24 h co-cultures with or without glycerol. A high C. jejuni growth was observed in cultures without glycerol. In contrast, C. jejuni growth decreased from 7.3 ± 0.1 log CFU/mL to below detection limit (1 log CFU/mL) during co-cultures added with 28 mM glycerol. This bactericidal effect could be attributed to in situ reuterin production. The low MIC observed and the high sensitivity towards in situ produced reuterin suggests L. reuteri combined with glycerol, as a possible intervention option to reduce Campylobacter in the food chain. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000396516Publication status
publishedExternal links
Journal / series
MicroorganismsVolume
Pages / Article No.
Publisher
MDPISubject
Acrolein; reuterin; antimicrobial; Lactobacillus reuteri; CampylobacterOrganisational unit
03626 - Lacroix, Christophe / Lacroix, Christophe
More
Show all metadata
Citations
Cited 24 times in
Web of Science
Cited 24 times in
Scopus
ETH Bibliography
yes
Altmetrics