Show simple item record

dc.contributor.author
Naito, Tomoaki
dc.contributor.author
Mulet, Céline
dc.contributor.author
De Castro, Christina
dc.contributor.author
Molinaro, Antonio
dc.contributor.author
Saffarian, Azadeh
dc.contributor.author
Nigro, Giulia
dc.contributor.author
Bérard, Marion
dc.contributor.author
Clerc, Melanie
dc.contributor.author
Pedersen, Amy B.
dc.contributor.author
Sansonetti, Phillipe J.
dc.contributor.author
Pédron, Thierry
dc.date.accessioned
2020-03-12T14:11:15Z
dc.date.available
2020-03-11T09:36:32Z
dc.date.available
2020-03-12T14:09:44Z
dc.date.available
2020-03-12T14:11:15Z
dc.date.issued
2017
dc.identifier.issn
2150-7511
dc.identifier.issn
2161-2129
dc.identifier.other
10.1128/mbio.01680-17
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/404304
dc.identifier.doi
10.3929/ethz-b-000404304
dc.description.abstract
We identified a crypt-specific core microbiota (CSCM) dominated by strictly aerobic, nonfermentative bacteria in murine cecal and proximal colonic (PC) crypts and hypothesized that, among its possible functions, it may affect epithelial regeneration. In the present work, we isolated representative CSCM strains using selective media based upon our initial 16S rRNA-based molecular identification (i.e., Acinetobacter, Delftia, and Stenotrophomonas). Their tropism for the crypt was confirmed, and their influence on epithelial regeneration was demonstrated in vivo by monocolonization of germfree mice. We also showed that lipopolysaccharide (LPS), through its endotoxin activity, was the dominant bacterial agonist controlling proliferation. The relevant molecular mechanisms were analyzed using colonic crypt-derived organoids exposed to bacterial sonicates or highly purified LPS as agonists. We identified a Toll-like receptor 4 (TLR4)-dependent program affecting crypts at different stages of epithelial differentiation. LPS played a dual role: it repressed cell proliferation through RIPK3-mediated necroptosis of stem cells and cells of the transit-amplifying compartment and concurrently enhanced cell differentiation, particularly the goblet cell lineage.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Microbiology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
homeostasis
en_US
dc.subject
intestinal stem cells
en_US
dc.subject
LPS
en_US
dc.subject
necroptosis
en_US
dc.title
Lipopolysaccharide from Crypt-Specific Core Microbiota Modulates the Colonic Epithelial Proliferation-to-Differentiation Balance
en_US
dc.type
Journal Article
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-10-17
ethz.journal.title
mBio
ethz.journal.volume
8
en_US
ethz.journal.issue
5
en_US
ethz.pages.start
e01680-17
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::09583 - Sunagawa, Shinichi / Sunagawa, Shinichi
ethz.identifier.orcidWorkCode
70368292
ethz.date.deposited
2020-03-11T09:36:42Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-03-12T14:09:55Z
ethz.rosetta.lastUpdated
2021-02-15T08:44:39Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Lipopolysaccharide%20from%20Crypt-Specific%20Core%20Microbiota%20Modulates%20the%20Colonic%20Epithelial%20Proliferation-to-Differentiation%20Balance&rft.jtitle=mBio&rft.date=2017&rft.volume=8&rft.issue=5&rft.spage=e01680-17&rft.issn=2150-7511&2161-2129&rft.au=Naito,%20Tomoaki&Mulet,%20C%C3%A9line&De%20Castro,%20Christina&Molinaro,%20Antonio&Saffarian,%20Azadeh&rft.genre=article&article&rft_id=info:doi/10.1128/mbio.01680-17&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record