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dc.contributor.author
Montellese, Christian
dc.contributor.author
van den Heuvel, Jasmin
dc.contributor.author
Ashiono, Caroline
dc.contributor.author
Dörner, Kerstin
dc.contributor.author
Melnik, André
dc.contributor.author
Jonas, Stefanie
dc.contributor.author
Zemp, Ivo
dc.contributor.author
Picotti, Paola
dc.contributor.author
Gillet, Ludovic C.
dc.contributor.author
Kutay, Ulrike
dc.date.accessioned
2020-03-26T10:04:36Z
dc.date.available
2020-03-26T02:55:30Z
dc.date.available
2020-03-26T10:04:36Z
dc.date.issued
2020
dc.identifier.issn
2050-084X
dc.identifier.other
10.7554/eLife.54435
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/406660
dc.identifier.doi
10.3929/ethz-b-000406660
dc.description.abstract
Establishment of translational competence represents a decisive cytoplasmic step in the biogenesis of 40S ribosomal subunits. This involves final 18S rRNA processing and release of residual biogenesis factors, including the protein kinase RIOK1. To identify novel proteins promoting the final maturation of human 40S subunits, we characterized pre-ribosomal subunits trapped on RIOK1 by mass spectrometry, and identified the deubiquitinase USP16 among the captured factors. We demonstrate that USP16 constitutes a component of late cytoplasmic pre-40S subunits that promotes the removal of ubiquitin from an internal lysine of ribosomal protein RPS27a/eS31. USP16 deletion leads to late 40S subunit maturation defects, manifesting in incomplete processing of 18S rRNA and retarded recycling of late-acting ribosome biogenesis factors, revealing an unexpected contribution of USP16 to the ultimate step of 40S synthesis. Finally, ubiquitination of RPS27a appears to depend on active translation, pointing at a potential connection between 40S maturation and protein synthesis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
eLife Sciences Publications
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
USP16 counteracts mono-ubiquitination of RPS27a and promotes maturation of the 40S ribosomal subunit
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-03-04
ethz.journal.title
eLife
ethz.journal.volume
9
en_US
ethz.pages.start
e54435
en_US
ethz.size
27 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Ribosome synthesis in mammalian cells
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Cambridge
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03543 - Kutay, Ulrike / Kutay, Ulrike
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03927 - Picotti, Paola / Picotti, Paola
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03543 - Kutay, Ulrike / Kutay, Ulrike
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03927 - Picotti, Paola / Picotti, Paola
ethz.grant.agreementno
166565
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2020-03-26T02:55:34Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-03-26T10:04:48Z
ethz.rosetta.lastUpdated
2021-02-15T09:40:25Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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